Free shipping on all orders over $ 500

Cisplatin

Cat. No. M2223
Cisplatin Structure
Synonym:

cisplatinum, CDDP

Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
100mg USD 48  USD48 In stock
500mg USD 120  USD120 In stock
1g USD 190  USD190 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control
Biological Activity

Cisplatin is an inorganic platinum complex that inhibits DNA synthesis by forming DNA crosslinking agents, inactivates GPXs, reduces cell GSH and induces iron death in HCT116 and A549 cells.

Product Citations
Customer Product Validations & Biological Datas
Source Cell Cycle (2018 Jul). Figure 5. Cisplatin (Abmole Bioscience)
Method cellular proliferation assay
Cell Lines SiHa cells
Concentrations 5 μM
Incubation Time -
Results However, we found no synergy between Apatinib and cisplatin (CI ranged from 1.149 to 5.647 in SiHa, CI ranged from 0.85 to 1.682 in Hcc94) or carboplatin (CI ranged from 0.669 to 1.402 in SiHa and from 0.955 to 1.206 in Hcc94)
Source Cancer Biol Ther (2018). Figure 6. cisplatin (Abmole Bioscience, Shanghai, China)
Method Buformin synergizes with chemotherapy
Cell Lines C33A cells
Concentrations 1 μM
Incubation Time
Results In C33A cells, low-dose buformin (1μM) significantly enhanced the anti-proliferative effects when combined with cisplatin, paclitaxel, or 5-FU
Source J Korean Med Sci (2016). Figure 5. cisplatin, mitoxantrone and topotecan were all purchased from AbMole BioScience (Shanghai, China).
Method CCK8 Assay and ABCG2 ATPase assay
Cell Lines RCSC cells
Concentrations 0~10 μM
Incubation Time 72 h
Results MiR-3163 plays important roles in multidrug resistance (Table 1, Fig. 5A). Results showed that when miR-3163 was overexpressed in RCSCs, the ATPase activity of ABCG2 was significantly downregulated compared to RCSCs with Scramble (Fig. 5B).
Source J Korean Med Sci (2016). Figure 4. cisplatin, mitoxantrone and topotecan were all purchased from AbMole BioScience (Shanghai, China).
Method CCK8 Assay and flow cytometry analysis
Cell Lines RCSC cells
Concentrations 0~10 μM
Incubation Time 72 h
Results "A lower proliferation rate was observed in RCSCs transfected with miR-3163 Mimics using the CCK-8 assay (Fig. 4A).RCSCs treated with miR-3163 Mimics were more susceptible to cisplatin-induced apoptosis compared to those treated with Scramble (Fig.4B)."
Protocol
Cell Experiment
Cell lines A549-luc cell
Preparation method Cells were allowed to adhere for 24 hours and subsequently incubated with either PRINT-Platin or cisplatin at concentrations ranging from 9.8 nM to 80 µM (drug concentration) for 72 hours at 37 °C in a humidified 5 % CO2 atmosphere. After the incubation period, all media/particles were aspirated off cells. 100 µL fresh medium was added back to cells, followed by the addition of 100 µL CellTiter-Glo® Luminescent Cell Viability Assay reagent.
Concentrations 9.8 nM to 80 µM
Incubation time 72 h
Animal Experiment
Animal models healthy mice
Formulation pH-adjusted 0.9 wt% NaCl solution
Dosages 3 mg/kg
Administration vein injection
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight 300.05
Formula Cl2H6N2Pt
CAS Number 15663-27-1
Purity 99.39%
Solubility DMF: 12 mg/mL
Storage at -20°C
References

[1] Kai MP, et al. J Control Release. Evaluation of drug loading, pharmacokinetic behavior, and toxicity of a cisplatin-containing hydrogel nanoparticle.

[2] Wilmes A, et al. Toxicol In Vitro. Mechanism of cisplatin proximal tubule toxicity revealed by integrating transcriptomics, proteomics, metabolomics and biokinetics.

[3] Matthew D Hall, et al. Cancer Res. Say no to DMSO: dimethylsulfoxide inactivates cisplatin, carboplatin, and other platinum complexes

Related DNA/RNA Synthesis Products
Tempo

Tempo is a nitric oxide radical and a selective scavenging agent of ROS that disambiguates superoxides in catalytic cycles. Tempo can induce DNA strand breaking and can be used as an organic catalyst for the oxidation of primary alcohols to aldehydes. Tempo has mutagenic and antioxidant effects.

NSC 617145

NSC 617145 is a selective werner syndrome helicase (WRN) helicase inhibitor,IC50 The value is 230 nM. NSC 617145 inhibit WRN ATPase and induce double-strand rupture (DSB) and chromosomal abnormalities. NSC 617145 is selective to WRN and superior to BLM, FANCJ, ChlR1, RecQ, and UvrD helicases.

IMP-1088

IMP-1088 is a novel potent and selective blocker of N-myristoylation in cells. IMP-1088 is also a potent human N-myristoyltransferases NMT1 and NMT2 dual inhibitor with IC50s of <1 nM for HsNMT1 and HsNMT2.

Methoxyamine HCl

Methoxyamine is an orally bioavailable small molecule inhibitor with potential adjuvant activity. Methoxyamine covalently binds to apurinic/apyrimidinic (AP) DNA damage sites and inhibits base excision repair (BER), which may result in an increase in DNA strand breaks and apoptosis.

RK-33

RK-33 is a first-in-class, potent and selective DDX3 (RNA helicase) inhibitor, it binds to DDX3 and abrogates its activity.

  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Cisplatin, cisplatinum, CDDP supplier, DNA/RNA Synthesis, inhibitors

Contact Us

Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2020 AbMole BioScience. All Rights Reserved.