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Cisplatin

Cat. No. M2223
Cisplatin Structure
Synonym:

cisplatinum, CDDP

Size Price Availability Quantity
300mg USD 100 In stock
1g USD 280 In stock
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Quality Control
Biological Activity

Cisplatin is an inorganic compound that is widely used for the treatment of a variety of tumors. Cisplatin induces apoptosis via p53-dependent and -independent mechanisms. Cisplatin inhibits X-linked inhibitor of apoptosis protein (XIAP) expression and activates caspase-3.

Protocol
Cell Experiment
Cell lines A549-luc cell
Preparation method Cells were allowed to adhere for 24 hours and subsequently incubated with either PRINT-Platin or cisplatin at concentrations ranging from 9.8 nM to 80 µM (drug concentration) for 72 hours at 37 °C in a humidified 5 % CO2 atmosphere. After the incubation period, all media/particles were aspirated off cells. 100 µL fresh medium was added back to cells, followed by the addition of 100 µL CellTiter-Glo® Luminescent Cell Viability Assay reagent.
Concentrations 9.8 nM to 80 µM
Incubation time 72 h
Animal Experiment
Animal models healthy mice
Formulation pH-adjusted 0.9 wt% NaCl solution
Dosages 3 mg/kg
Administration vein injection
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 300.05
Formula Cl2H6N2Pt
CAS Number 15663-27-1
Purity >99%
Solubility DMSO 50 mg/mL
Storage at -20°C
Customer Product Validations & Biological Datas
Source J Korean Med Sci (2016). Figure 5. cisplatin, mitoxantrone and topotecan were all purchased from AbMole BioScience (Shanghai, China).
Method CCK8 Assay and ABCG2 ATPase assay
Cell Lines RCSC cells
Concentrations 0~10 μM
Incubation Time 72 h
Results MiR-3163 plays important roles in multidrug resistance (Table 1, Fig. 5A). Results showed that when miR-3163 was overexpressed in RCSCs, the ATPase activity of ABCG2 was significantly downregulated compared to RCSCs with Scramble (Fig. 5B).
Rating
Source J Korean Med Sci (2016). Figure 4. cisplatin, mitoxantrone and topotecan were all purchased from AbMole BioScience (Shanghai, China).
Method CCK8 Assay and flow cytometry analysis
Cell Lines RCSC cells
Concentrations 0~10 μM
Incubation Time 72 h
Results "A lower proliferation rate was observed in RCSCs transfected with miR-3163 Mimics using the CCK-8 assay (Fig. 4A).RCSCs treated with miR-3163 Mimics were more susceptible to cisplatin-induced apoptosis compared to those treated with Scramble (Fig.4B)."
Rating
Product Citations
References

Evaluation of drug loading, pharmacokinetic behavior, and toxicity of a cisplatin-containing hydrogel nanoparticle.
Kai MP, et al. J Control Release. 2015 Apr 28;204:70-7. PMID: 25744827.

Mechanism of cisplatin proximal tubule toxicity revealed by integrating transcriptomics, proteomics, metabolomics and biokinetics.
Wilmes A, et al. Toxicol In Vitro. 2015 Dec 25;30(1 Pt A):117-27. PMID: 25450742.

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Keywords: Cisplatin, cisplatinum, CDDP supplier, DNA/RNA Synthesis, inhibitors

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