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CAY10603

Cat. No. M3888

CAY10603 Structure

Synonym: Isox

Size Price Availability Quantity
2mg USD 120 In stock
5mg USD 240 In stock
10mg USD 360 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

CAY10603 prevents the growth of several pancreatic cancer cell lines (IC50 = 0.1-1 μM). CAY10603 shows potent antiproliferative activity against pancreatic cancer cell lines with IC50 of <1 μM. CAY10603 can be used as a new molecular probe in exploring HDAC biology.

Protocol
Cell Experiment
Cell lines BxPc-3, HupT3, Mia Paca-2, Panc 04.03, and SU 86.86 cells
Preparation method Growing the pancreatic cancer cell lines BxPc-3, HupT3, Mia Paca-2, Panc 04.03, and SU 86.86 in medium (DMEM or RPMI) which contains 10% fetal calf serum and l-glutamine. Plating pancreatic cancer cells out in duplicate into 6 wells of a 96-well microtiter plate. Four hours post plating, treting individual wells with diluent (DMSO) or varying concentrations of SAHA or the indicated HDACIs from a concentration of 1 nM to 50 mM.Measuring cytotoxicity at time “0”, and 72 h post treatment using the colorimetric MTT assay according to the manufacturer’s suggestions. Using XLfit to calculate the IC50 values .
Concentrations ~50 μM
Incubation time 72 hours
Animal Experiment
Animal models
Formulation
Dosages
Administration
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 446.5
Formula C22H30N4O6
CAS Number 1045792-66-2
Purity >99%
Solubility DMSO
Storage at -20°C
References

Use of the nitrile oxide cycloaddition (NOC) reaction for molecular probe generation: a new class of enzyme selective histone deacetylase inhibitors (HDACIs) showing picomolar activity at HDAC6.
Kozikowski AP, et al. J Med Chem. 2008 Aug 14;51(15):4370-3. PMID: 18642892.

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: CAY10603, Isox supplier, HDAC, inhibitors

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