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BMS-777607

Cat. No. M1682
BMS-777607 Structure
Size Price Availability Quantity
10mg USD 120 In stock
50mg USD 380 In stock
100mg USD 650 In stock
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Quality Control
Biological Activity

BMS-777607 is a selective and potent small-molecule met kinase inhibitor. Overexpression of c-Met receptor tyrosine kinase has been highly associated with prostate cancer progression and metastasis. BMS-777607 suppressed HGF-stimulated cell migration and invasion in a dose-dependent fashion (IC50 < 0.1 μmol/L). Mechanistically, nanomolar doses of BMS 777607 potently blocked HGF-stimulated c-Met autophosphorylation and downstream activation of Akt and extracellular signal-regulated kinase.

Protocol
Cell Experiment
Cell lines PC-3 cell line
Preparation method Cell proliferation and cell death Cells were seeded in a 96-well plate at a density of 5 × 103 cells per well and exposed to serial dilutions of BMS-777607 for 1 hour. HGF (25 ng/mL) was then added and the cells were incubated (drug plus HGF) for a period of 96 hours. At the end of the treatment period, the tumor cells were exposed to WST-8 (MTT assay reagent) in a Cell Counting kit (Donjindo Molecular Technologies) according to the manufacturer's instruction, and absorbance was determined at 450 nm colorimetrically. Cell proliferation (%) was calculated as the ratio of absorbance from treated sample to the nontreated control. Cell death was examined by trypan blue exclusion, and positively stained (dead) cells were scored using a hemocytometer.
Concentrations 0, 0.1, 0.3, 1, 3, 10 μM
Incubation time 96 h
Animal Experiment
Animal models C3H/HeJ mice with KHT lung tumor model
Formulation DMSO
Dosages 25mg/kg daily
Administration oral gavage
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 512.89
Formula C25H19ClF2N4O4
CAS Number 1196681-44-3
Purity >99%
Solubility DMSO 40 mg/mL
Storage at -20°C
References

Constitutively active c-Met kinase in PC-3 cells is autocrine-independent and can be blocked by the Met kinase inhibitor BMS-777607.
Dai et al. BMC Cancer. 2012 May 28;12(1):198. PMID: 22639908.

Impact of the small molecule Met inhibitor BMS-777607 on the metastatic process in a rodent tumor model with constitutive c-Met activation.
Dai et al. Clin Exp Metastasis. 2012 Mar;29(3):253-61. PMID: 22286523.

BMS-777607, a small-molecule met kinase inhibitor, suppresses hepatocyte growth factor-stimulated prostate cancer metastatic phenotype in vitro.
Dai et al. Mol Cancer Ther. 2010 Jun;9(6):1554-61. PMID: 20515943.

Discovery of N-(4-(2-amino-3-chloropyridin-4-yloxy)-3-fluorophenyl)-4-ethoxy-1-(4-fluorophenyl)-2-oxo-1,2-dihydropyridine-3-carboxamide (BMS-777607), a selective and orally efficacious inhibitor of the Met kinase superfamily.
Schroeder et al. J Med Chem. 2009 Mar 12;52(5):1251-4. PMID: 19260711.

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: BMS-777607 supplier, c-Met, inhibitors

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