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BMS-5

Cat. No. M6145
BMS-5 Structure
Synonym:

LIMKI 3

Size Price Availability Quantity
5mg USD 100 In stock
10mg USD 160 In stock
50mg USD 450 In stock
100mg USD 810 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

COCs exhibited different diffusion situation between control and treatment group: the diffusion of 200 µM LIMKi 3 treated-COCs was significantly weaker than the control group. In the standard of COCs surrounded by the cumulus cell layers, we counted the proportion of diffusion and found that the rate of good diffusion was decreased significantly (40.05 ± 3.03% vs. 20.50 ± 4.05%; p < 0.05). Oocytes were then obtained from COCs after hyaluronidase treatment, we found that few oocytes extruded the polar body after 200 µM LIMKi 3 treatment.

Protocol
Cell Experiment
Cell lines COCs
Preparation method For LIMK1/2 inhibitor treatment, stock LIMKi 3 (50 mM in dimethylsulfoxide (DMSO)) was diluted in M199 to final concentrations of 50, 100, 150 and 200 µM. A control group was cultured in DMSO at the same relative concentration of solvent. COCs were cultured with LIMKi 3 to evaluate its effects on oocyte maturation. COCs were denuded of their cumulus cells by gentle pipetting with 0.1% (w/v) hyaluronidase.
Concentrations 200 µM
Incubation time 44 h
Animal Experiment
Animal models NF1+/− mice
Formulation DMEM × 1 containing 5% FCS
Dosages 0, 25 or 50 μM
Administration i.v.
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 431.29
Formula C17H14Cl2F2N4OS
CAS Number 1338247-35-0
Purity >99%
Solubility 34 mg/mL in DMSO
Storage at -20°C
References

LIMK1/2 inhibitor LIMKi 3 suppresses porcine oocyte maturation.
Jia RX, et al. PeerJ. 2016 Oct 12;4:e2553. PMID: 27761340.

Computer-based identification of a novel LIMK1/2 inhibitor that synergizes with salirasib to destabilize the actin cytoskeleton.
Mashiach-Farkash E, et al. Oncotarget. 2012 Jun;3(6):629-39. PMID: 22776759.

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: BMS-5, LIMKI 3 supplier, inhibitors

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