Free shipping on all orders over $ 500

10-Hydroxycamptothecin

Cat. No. M7507
10-Hydroxycamptothecin Structure
Synonym:

Hydroxy Camptothecine

Size Price Availability Quantity
100mg USD 145  USD145 In stock
Free Delivery on orders over USD 500 Bulk Inquiry?

Quality Control & Documentation
Biological Activity

In vitro: 10-HCPT has an inhibitory effect on phosphorylation of histone H1 and H3 in murine hepatoma cells and also exhibits a differentiation inducing effect in human HepG2 cells. 10-HCPT inhibits the cell growth, reduces the cell viability and disturbs the cell cycle distribution of human colon cancer cell line Colo 205. It induces cell cycle arrest in the G2/M phase.

In vivo: 10-HCPT can be safely orally administered and, alternatively, a low-dose long-term treatment. Prolonged elimination of HCPT in vivo may have a significant impact on its therapeutic effects. HCPT is metabolized to its carboxylate form and glucuronides. Dose-dependent toxicity is observed with i.v. administration of HCPT.

Protocol (for reference only)
Cell Experiment
Cell lines Colo 205 cells
Preparation method Colo 205 cells (5×105) (ATCC:CCL-222) are seeded in 25T flasks overnight and then treated without (control) and with 5, 10, 15 or 20 nM of HCPT, respectively. After treatment for 24-120 h, cells are harvested by trypsin-EDTA and then centrifuged at 1,500 rpm for 5 min at 4˚C. The cell pellet is resuspended in culture medium containing 0.04% trypan blue and the viable cells are enumerated by a hemocytometer.
Concentrations 5, 10, 15 or 20 nM
Incubation time 24-120 h
Animal Experiment
Animal models BALB/c-nude mice
Formulation propylene glycol
Dosages 1, 2.5, 5, 7.5 mg/kg
Administration oral administration
Chemical Information
Molecular Weight 364.35
Formula C20H16N2O5
CAS Number 64439-81-2
Solubility (25°C) 29 mg/mL in DMSO
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Chen Y, et al. J Nanosci Nanotechnol. Liposomes Encapsulating 10-Hydroxycamptothecin-Cyclodextrin Complexes and Their In Vitro Anti-Tumor Activities.

[2] Yang L, et al. J Biomed Nanotechnol. High Drug Payload 10-Hydroxycamptothecin Nanosuspensions Stabilized by Cholesterol-PEG: In Vitro and In Vivo Investigation.

Related Topoisomerase Products
ICRF-193

ICRF-193 is an TopoII inhibitor.

Topoisomerase II inhibitor 15

Topoisomerase II inhibitor 15 is a Topoisomerase II inhibitor.

Topoisomerase II inhibitor 13

Topoisomerase II inhibitor 13 is a topoisomerase II (Topo II) inhibitor.

ICRF-196

ICRF-196 is an racemic mixture of the (S,S)- and (R,R)-isomers of ICRF-193.

Cholesteryl hemisuccinate

Cholesteryl hemisuccinate is a with hepatoprotective an anticancer activity.

  Catalog
Abmole Inhibitor Catalog




Keywords: 10-Hydroxycamptothecin, Hydroxy Camptothecine supplier, Topoisomerase, inhibitors, activators


Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.