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10-Hydroxycamptothecin

Cat. No. M3924

10-Hydroxycamptothecin Structure

Synonym: NSC 107124

Size Price Availability Quantity
10mg USD 60 In stock
50mg USD 150 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

10-hydroxy-Camptothecin is an inhibitor of topoisomerase I originally isolated from the Chinese tree C. acuminata. 10-Hydroxycamptothecin is a member of the camptothecin family that demonstrates less toxicity than its parent compound. (S)-10-hydroxy-Camptothecin has strong anti-tumor activity against a wide range of experimental tumors including L1210 leukemia cells (IC50 = 1.15 μM). In vitro treatment of human HepG2 cells with 5-20 μM (S)-10-hydroxy-camptothecin results in cell cycle arrest at the G2/M phase.

Protocol
Cell Experiment
Cell lines MDA-231, and BT-20 cells
Preparation method Exposing cells to various concentrations of 10-Hydroxycamptothecin for 72 hours. Monitoring cell growth by the standard MTT assay.
Concentrations Dissolved in DMSO, final concentrations ~50 μM
Incubation time 72 hours
Animal Experiment
Animal models BALB/c-nu mice transplanted subcutaneously with Colo 205 cells
Formulation Dissolved in propylene glycol
Dosages ~7.5 mg/kg
Administration Orally once per two days
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 364.35
Formula C20H16N2O5
CAS Number 19685-09-7
Purity >99%
Solubility DMSO 5mg/ml
DMF
methanol
Storage at -20°C
References

[Studies on the effects of cucurbit (n = 7) uril on the physicohemical properties and anticancer activity of 10-hydroxycamptothecin].
Lü Y, et al. Guang Pu Xue Yu Guang Pu Fen Xi. 2014 Jun;34(6):1610-4. PMID: 25358173.

Anticancer effects of low-dose 10-hydroxycamptothecin in human colon cancer.
Ping YH, et al. Oncol Rep. 2006 May;15(5):1273-9. PMID: 16596197.

DNA topoisomerase I as a site of action for 10-hydroxycamptothecin in human promyelocytic leukemia cells.
Ling YH, et al. Cancer Biochem Biophys. 1990 Jan;11(1):23-30. PMID: 2159843.

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: 10-Hydroxycamptothecin, NSC 107124 supplier, Topoisomerase, inhibitors

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