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10-Hydroxycamptothecin

Cat. No. M7507
10-Hydroxycamptothecin Structure
Synonym:

Hydroxy Camptothecine

Size Price Availability Quantity
100mg USD 145 In stock
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Quality Control
  • Current batch:
  • Purity >98%
  • COA
  • MSDS
Biological Activity

In vitro: 10-HCPT has an inhibitory effect on phosphorylation of histone H1 and H3 in murine hepatoma cells and also exhibits a differentiation inducing effect in human HepG2 cells. 10-HCPT inhibits the cell growth, reduces the cell viability and disturbs the cell cycle distribution of human colon cancer cell line Colo 205. It induces cell cycle arrest in the G2/M phase.

In vivo: 10-HCPT can be safely orally administered and, alternatively, a low-dose long-term treatment. Prolonged elimination of HCPT in vivo may have a significant impact on its therapeutic effects. HCPT is metabolized to its carboxylate form and glucuronides. Dose-dependent toxicity is observed with i.v. administration of HCPT.

Protocol
Cell Experiment
Cell lines Colo 205 cells
Preparation method Colo 205 cells (5×105) (ATCC:CCL-222) are seeded in 25T flasks overnight and then treated without (control) and with 5, 10, 15 or 20 nM of HCPT, respectively. After treatment for 24-120 h, cells are harvested by trypsin-EDTA and then centrifuged at 1,500 rpm for 5 min at 4˚C. The cell pellet is resuspended in culture medium containing 0.04% trypan blue and the viable cells are enumerated by a hemocytometer.
Concentrations 5, 10, 15 or 20 nM
Incubation time 24-120 h
Animal Experiment
Animal models BALB/c-nude mice
Formulation propylene glycol
Dosages 1, 2.5, 5, 7.5 mg/kg
Administration oral administration
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 364.35
Formula C20H16N2O5
CAS Number 64439-81-2
Purity >98%
Solubility 29 mg/mL in DMSO
Storage at -20°C
References

Liposomes Encapsulating 10-Hydroxycamptothecin-Cyclodextrin Complexes and Their In Vitro Anti-Tumor Activities.
Chen Y, et al. J Nanosci Nanotechnol. 2015 May;15(5):3786-95. PMID: 26505006.

High Drug Payload 10-Hydroxycamptothecin Nanosuspensions Stabilized by Cholesterol-PEG: In Vitro and In Vivo Investigation.
Yang L, et al. J Biomed Nanotechnol. 2015 Apr;11(4):711-21. PMID: 26310077.

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: 10-Hydroxycamptothecin, Hydroxy Camptothecine supplier, Topoisomerase, inhibitors

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