Wnt-C59 (C59) is a PORCN inhibitor for Wnt3A-mediated activation of a multimerized TCF-binding site driving luciferase with IC50 of 74 pM.
|Source||Stem Cells Transl Med (2016). Figure 4. Wnt-C59|
|Cell Lines||human iPSCs|
|Incubation Time||4 or 5 days|
|Results||qPCR analyses revealed that neural aggregates differentiated with C59 contained more cells with rostral identity compared with DKK1 or XAV|
|Cell lines||NMuMG (NMG) and MDA-MB 231 cells|
|Preparation method||Cells are seeded at a concentration of 4×103/well in 100 μL culture medium containing various amouts of Wnt-C59 and incubated for 48 hours at 37 ℃. Add 10 μL/well cell proliferation reagent and incubate for 4 hours at 37 ℃. Shake thoroughly for 1 min on a shaker. Measure the absorbance of the samples in a microplate (ELISA) reader.|
|Incubation time||48 hours|
|Animal models||Female nude mice orthotopically transplanted with independent MMTV-WNT1 tumors|
|Formulation||0.5% methylcellulose and 0.1% Tween-80|
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
|Solubility||DMSO 56 mg/mL|
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