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Wnt-C59

Cat. No. M3131
Wnt-C59  Structure
Size Price Availability Quantity
5mg USD 90 In stock
10mg USD 150 In stock
50mg USD 550 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

Wnt-C59 (C59) is a PORCN inhibitor for Wnt3A-mediated activation of a multimerized TCF-binding site driving luciferase with IC50 of 74 pM.

Protocol
Cell Experiment
Cell lines NMuMG (NMG) and MDA-MB 231 cells
Preparation method Cells are seeded at a concentration of 4×103/well in 100 μL culture medium containing various amouts of Wnt-C59 and incubated for 48 hours at 37 ℃. Add 10 μL/well cell proliferation reagent and incubate for 4 hours at 37 ℃. Shake thoroughly for 1 min on a shaker. Measure the absorbance of the samples in a microplate (ELISA) reader.
Concentrations
Incubation time 48 hours
Animal Experiment
Animal models Female nude mice orthotopically transplanted with independent MMTV-WNT1 tumors
Formulation 0.5% methylcellulose and 0.1% Tween-80
Dosages 10 mg/kg
Administration orally
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 379.45
Formula C25H21N3O
CAS Number 1243243-89-1
Purity >99%
Solubility DMSO 60 mg/mL
Storage at -20°C
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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Wnt-C59 supplier, Wnt/beta-catenin, inhibitors

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