TPCK (L-1-Tosylamido-2-phenylethyl chloromethyl ketone; L-TPCK) is a potent serine protease inhibitor that reacts with the retinoblastoma protein (RB)-binding core of HPV-18 E7 protein and abolish its RB-binding capability. TPCK is able to modify E7 protein in live keratinocytes following its addition to the culture medium.
Molecular Weight | 351.85 |
Formula | C17H18ClNO3S |
CAS Number | 402-71-1 |
Form | Solid |
Solubility (25°C) | DMSO ≥ 100 mg/mL |
Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
Related Serine/Threonine Protease Products |
---|
HC-7366
HC-7366 is an orally bioavailable and efficacious GCN2 kinase activator. |
Phe-Pro-Ala-pNA
Phe-Pro-Ala-pNA is a chromogenic substrate of tripeptidyl peptidase. |
Dansyl-Glu-Gly-Arg-Chloromethylketone
Dansyl-Glu-Gly-Arg-Chloromethylketone is a protease inhibitor, and inhibits serine/threonine proteases. |
Fluorescent Substrate for Subtillsin
Fluorescent Substrate for Subtillsin can be used to detect the enzyme activity of subtillsin. |
N-Benzoyl-L-tyrosine p-nitroanilide
N-Benzoyl-L-tyrosine p-nitroanilide is a chymotrypsin substrate. |
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.