We have demonstrated, to the best of our knowledge for the first time, the effects of short (1-day) and prolonged (2-weeks, 10-weeks) in vivo administrations of testosterone enanthate (TE) on molecular markers of pituitary functionality as well as prolactin (PRL) and cAMP signaling in Leydig cells.
|Cell lines||Leydig cells|
|Preparation method||For in vitro experiments the primary cultures of purified Leydig cells were isolated from undisturbed rats and plated for monitoring genes expression. After 2 h of recovery, the culture medium was trashed and the Leydig cells were incubated for 20 h without (CUT, untreated (control) cells) or with test osterone (T, 20 μM ) or cyproterone-acetate (CPA, 100 μM ) or combination CPA (100 μM ) + T (20 μM ), or in presence of hCG (5 192 and 50 ng/ml) as a positive control.|
|Incubation time||20 h|
|Animal models||male Wistar rats|
|Dosages||0.5mg/100g or 2.5 mg/100g|
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
|Solubility||10 mM in DMSO|
Prolonged in vivo administration of testosterone-enanthate, the widely used and abused anabolic androgenic steroid, disturbs prolactin and cAMP signaling in Leydig cells of adult rats.
Bjelic MM, et al. J Steroid Biochem Mol Biol. 2015 May;149:58-69. PMID: 25603467.
Molecular adaptations of testosterone-producing Leydig cells during systemic in vivo blockade of the androgen receptor.
Bjelic MM, et al. Mol Cell Endocrinol. 2014 Oct;396(1-2):10-25. PMID: 25153259.
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