T7 RNA polymerase is a DNA-dependent RNA polymerase that specifically recognizes the T7 promoter sequence (5' -TaatacgactCactatagggaga-3 '). T7 RNA polymerase can catalyze NTP incorporation of single or double stranded DNA template downstream of T7 promoter to synthesize RNA complementary to the downstream DNA template of T7 promoter. Linear plasmids containing T7 promoters or PCR products containing T7 promoters can be used as templates for RNA synthesis in vitro. It can be used to synthesize various RNA precursors including mRNA, siRNA, gRNA and so on.
Source: This product is expressed by Escherichia coli and the expressed gene is phage T7 RNA polymerase gene
Storage and stability: stored at -20℃, valid for 24 months
Advantages: Highly specific recognition of T7 promoter, no nuclease, no protease
| Storage | -20°C |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[1] Luiz F M Passalacqua, et al. RNA. Single-pass transcription by T7 RNA polymerase
[4] S Tabor. Curr Protoc Mol Biol. Expression using the T7 RNA polymerase/promoter system
[5] Rui Sousa, et al. Prog Nucleic Acid Res Mol Biol. T7 RNA polymerase
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