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SU5402

Cat. No. M2194
SU5402 Structure
Size Price Availability Quantity
5mg USD 70 In stock
10mg USD 95 In stock
50mg USD 350 In stock
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Quality Control
Biological Activity

SU5402 is a potent and selective small molecular vascular endothelial growth factor receptor (VEGFR) and fibroblast growth factor receptor (FGFR) inhibitor. SU5402 inhibits embryonic left-right determination and exhibits potent anticancer activity in vitro and in vivo.

Protocol
Cell Experiment
Cell lines KMS11, UTMC-2, LP1, OPM1, OPM2, KHM11, JJN3, RPMI8226, EJM, K620, H929, U266 and OCI-My5 cell lines
Preparation method KMS11 (provided by MasayoshiNamba), UTMC-2, LP1, OPM1, OPM2, KHM11, JJN3, RPMI8226, EJM, K620, H929 (provided by Leif Bergsagel), U266 and OCI-My5 (provided by Robert Hawley) were grown in Iscove’s modified Dulbecco’s medium (IMDM) supplemented with 10% fetal calf serum (FCS) and 1% Gibco antimycotic/antibiotic (Invitrogen Canada, Ontario, Canada). For proliferation assays, cells were washed and plated in triplicate in IMDM plus 5% FCS and incubated with various concentrations of SU5402. Cells were then analysed by MTT [3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide] assay (Boehringer Mannheim, Mannheim, Germany) according to the manufacturer’s protocol.
Concentrations 0, 2,10µM
Incubation time 48 h
Animal Experiment
Animal models Spinal cords lesions of zebrafish
Formulation DMSO
Dosages 1 μl of 5 μg/μl
Administration injection
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 296.32
Formula C17H16N2O3
CAS Number 215543-92-3
Purity 100.00%
Solubility DMSO
Storage at -20°C
Customer Product Validations & Biological Datas
Source Stem Cell Reports (2017). SU5402, Figure1 . (AbMole Bioscience)
Method
Cell Lines The mouse ESC lines J1 (ATCC SCRC-1010), CCE, and Jmjd2c KO
Concentrations 10 μM
Incubation Time
Results FGFR inhibition (FGFRi) by SU5402 had little effect compared with LIF (Figure 1A). A two-inhibitor combination (PD0325901 and CHIR99021) and a threeinhibitor combination (PD0325901, CHIR99021, and SU5402) produced robust AP staining of ESCs with a compact colony morphology.
Rating
Product Citations
References

Nectin-1 binds and signals through the fibroblast growth factor receptor.
Bojesen KB, et al. J Biol Chem. 2012 Oct 26;287(44):37420-33. PMID: 22955284.

Characterization and expressional analysis of Dleu7 during Xenopus tropicalis embryogenesis.
Zhu X, et al. Gene. 2012 Nov 1;509(1):77-84. PMID: 22939871.

A retrospective analysis of germline competence in rat embryonic stem cell lines.
Hirabayashi M, et al. Transgenic Res. 2013 Apr;22(2):411-6. PMID: 22875289.

FGF-induced vesicular release of Sonic hedgehog and retinoic acid in leftward nodal flow is critical for left-right determination.
Tanaka Y, et al. Nature. 2005 May 12;435(7039):172-7. PMID: 15889083.

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: SU5402 supplier, VEGFR/PDGFR, inhibitors

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