Ramipril (Altace) is an angiotensin-converting enzyme (ACE) inhibitor with an IC50 of 5 nM. It is used to treat hypertension and congestive heart failure. ACE inhibitors lower the production of angiotensin II, therefore relaxing arterial muscles while at the same time enlarging the arteries, allowing the heart to pump blood more easily, and increasing blood flow due to more blood being pumped into and through larger passageways.
|Cell lines||Human umbilical vein endothelial cells (HUVECs)|
|Preparation method||Pretreating the HUVECs with the active metabolites of Ramipril for 24 hours. Usinf a serum deprivation method to induce apoptosis in the presence of Ramipril for an additional 24 hours. Then determining the rate of apoptosis using flow cytometry with two makers annexinV fluorescein isothiocyanate (FITC+) and propidium iodide (PI).|
|Incubation time||24 hours|
|Animal models||Male spontaneously hypertensive rats|
|Formulation||Dissolved in distilled water by using gum arabic (10% w/v)|
|Administration||Gavage, every day|
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
|Solubility||DMSO 80 mg/mL|
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