Pluronic F-127 was used for fluorescent labeling of blood vessels, astrocytes, and neurons. Pluronic F-127 is frequently used with dye AM esters such as Indo-1 AM, Fura-2 AM, Calcein AM, Fluo-3 AM, Fluo-4 AM, etc. to improve their water solubility. Pluronic F-127 was used to coat a siliconized coverslip to hold an egg extract in a study. Pluronic F-127 was added to phosphate buffered saline (PBS), to lower unspecific cell and protein adhesion to a PDMS-based microfluidic device.
| CAS Number | 9003-11-6 |
| Solubility (25°C) | DMSO 200 mg/mL Water >110 mg/mL (May need warm to 40-50°C) |
| Storage | 2-8°C, protect from light |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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| WGA
Wheat Germ Agglutinin (WGA) selectively binds to glycoprotein and glycollipic n-acetylglucosamine (GlcNAc) and n-acetylnervine (sialic acid) residues, and can be used to label the cell membranes of mammalian cells, gram-positive bacteria and yeast. And the sacral membrane of the bone and heart. |
| Diphenyl Blue
Diphenyl Blue (Direct Blue 14) is a reference dye used as a group of azo dyes. |
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