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5(6)-CFDA

Cat. No. M13760
5(6)-CFDA Structure
Synonym:

5-(6)-Carboxyfluorescein diacetate; CFDA

Size Price Availability Quantity
50mg USD 96  USD96 In stock
100mg USD 165  USD165 In stock
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Quality Control & Documentation
Biological Activity

5(6)-CFDA (5-(6)-Carboxyfluorescein diacetate) is a cell-permeant, amine-reactive green fluorescent probe used to stain cells for analysis in applications. (λex: 492 nm; λem: 517 nm).

Chemical Information
Molecular Weight 460.39
CAS Number 124387-19-5
Solubility (25°C) DMSO 90 mg/mL
Storage -20°C, protect from light
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Iigo Terrn, et al. Methods Enzymol. CFSE dilution to study human T and NK cell proliferation in vitro

[2] A Bruce Lyons, et al. Curr Protoc Cytom. Flow cytometric analysis of cell division by dilution of CFSE and related dyes

[3] Edwin D Hawkins, et al. Nat Protoc. Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data

[4] Ben J C Quah, et al. Nat Protoc. Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester

[5] R E Petroski, et al. J Neurosci Methods. Selective labeling of embryonic neurons cultured on astrocyte monolayers with 5(6)-carboxyfluorescein diacetate (CFDA)

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Keywords: 5(6)-CFDA, 5-(6)-Carboxyfluorescein diacetate; CFDA supplier, Cell Staining, inhibitors, activators


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