5(6)-CFDA (5-(6)-Carboxyfluorescein diacetate) is a cell-permeant, amine-reactive green fluorescent probe used to stain cells for analysis in applications. (λex: 492 nm; λem: 517 nm).
Molecular Weight | 460.39 |
CAS Number | 124387-19-5 |
Solubility (25°C) | DMSO 90 mg/mL |
Storage | -20°C, protect from light |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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