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PF-562271

Cat. No. M2302
PF-562271 Structure
Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
10mM/1mL In DMSO USD 150 In stock
5mg USD 130 In stock
10mg USD 210 In stock
50mg USD 530 In stock
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Quality Control
Biological Activity

PF-562271 is a potent, ATP-competitive, reversible inhibitor of FAK and Pyk2 with IC50 of 1.5 nM and 14 nM, respectively.

Product Citations
Customer Product Validations & Biological Datas
Source AM J Resp Crit Care (2018). Figure E2. PF562271 (Abmole Bioscience)
Method qPCR
Cell Lines Lung fibroblasts
Concentrations 10 μM
Incubation Time 24 h
Results Focal Adhesion Kinase (PF562271), ALK5 (A83-01), or MTRF (CCG-100602) did not consistently restore fibroblast miR-29 levels on IPF-ECM
Source Mol Cancer Ther. (2015) Figure 5. PF-562271 (M2302, Abmole, shanghai, China)
Method western blotting, immunohistochemical staining(IHC)
Cell Lines SKOV3 cells
Concentrations
Incubation Time
Results NISCH suppressed tumor metastasis via inhibiting FAK signal transduction in vivo.
Source Mol Cancer Ther. (2015) Figure 5. PF-562271 (M2302, Abmole, shanghai, China)
Method western blotting, immunohistochemical staining(IHC)
Cell Lines SKOV3 cells
Concentrations
Incubation Time 72h
Results Nischarin attenuated cell invasion by inhibiting FAK signal transduction.
Protocol
Cell Experiment
Cell lines MPanc-96 or MAD08-608 cells
Preparation method Cell migration and invasion assays were performed using transwell migration chambers (BD Biosciences, Bedford, MA) as previously described (26–28). Conditioned media was prepared from subconfluent MPanc-96 or MAD08-608 cells by harvesting growth medium (containing 0.5% FBS) for two 24 h periods. The conditioned media was centrifuged to remove cellular debris and concentrated 3 fold using Amicon Ultra-15 3K centrifugal filter devices (Millipore, Billerica, MA). Mock conditioned media was prepared by concentrating 0.5% FBS in similar fashion. For proliferation assays, cells (1.5 × 105) were plated in 96-well plates in 1% FBS-containing media and cell number was measured using the CyQuant assay (Invitrogen, Grand Island, NY).
Concentrations 0~1µM
Incubation time 48h
Animal Experiment
Animal models Orthotopic tumor model (Six-week old male athymic nude mice)
Formulation saline
Dosages 33 mg/kg twice/day
Administration by gastric lavage
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 507.49
Formula C21H20F3N7O3S
CAS Number 717907-75-0
Purity >99%
Solubility DMSO 80 mg/mL
Storage at -20°C
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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: PF-562271 supplier, FAK, inhibitors

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