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Oligomycin A

Cat. No. M1997

Oligomycin A Structure

Synonym: BRN 5702132, RP-32705

Size Price Availability Quantity
2mg USD 200 In stock
5mg USD 300 In stock
10mg USD 450 In stock
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Quality Control
Biological Activity

Oligomycin A inhibits membrane bound mitochondrial ATP synthase (ATPase), proton channel (pump, FO subunit) blockers to the cell membrane. Oligomycin A also inhibits oxidative phosphorylation and the electron transport chain. Oligomycin A decreased cytoplasmic ATP contents and disassembled metaphase II oocyte spindles when introduced to mouse oocytes. Oligomycin A is used to prevent state 3 (phosphorylating) respiration in oxidative phosphorylation research.

Protocol
Cell Experiment
Cell lines H1229 cell
Preparation method ATP and Oligomycin Dose-Response Growth Measurement. Cellular ATP changes are measured by CellTiter-Glo reagent. To measure oligomycin dose-response curves, the cells are plated in 96-well plates at about 400–500 cells/well in 100 μl of culture, dosed the next day, and grown for 4 additional days followed by assaying with Cell-Titer-Glo reagent. The dose-response curves are plotted with nonlinear regression analysis of GraphPad Prism.
Concentrations 100 ng/ml
Incubation time 1 h
Animal Experiment
Animal models
Formulation
Dosages
Administration
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 791.06
Formula C45H74O11
CAS Number 579-13-5
Purity >99%
Solubility DMSO
Storage at -20°C
References

Metabolic stress boosts humoral responses in vivo independently of inflammasome and inflammatory reaction.
Andris F, et al. J Immunol. 2011 Feb 15;186(4):2245-53. PMID: 21248260.

Deficit of mitochondria-derived ATP during oxidative stress impairs mouse MII oocyte spindles.
Zhang X, et al. Cell Res. 2006 Oct;16(10):841-50. PMID: 16983401.

Mitochondria in eosinophils: functional role in apoptosis but not respiration.
Peachman KK, et al. Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1717-22. PMID: 11172017.

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Oligomycin A, BRN 5702132, RP-32705 supplier, ATPase, inhibitors

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