Synonym: BRN 5702132, RP-32705
Oligomycin A inhibits membrane bound mitochondrial ATP synthase (ATPase), proton channel (pump, FO subunit) blockers to the cell membrane. Oligomycin A also inhibits oxidative phosphorylation and the electron transport chain. Oligomycin A decreased cytoplasmic ATP contents and disassembled metaphase II oocyte spindles when introduced to mouse oocytes. Oligomycin A is used to prevent state 3 (phosphorylating) respiration in oxidative phosphorylation research.
|Cell lines||H1229 cell|
|Preparation method||ATP and Oligomycin Dose-Response Growth Measurement. Cellular ATP changes are measured by CellTiter-Glo reagent. To measure oligomycin dose-response curves, the cells are plated in 96-well plates at about 400–500 cells/well in 100 μl of culture, dosed the next day, and grown for 4 additional days followed by assaying with Cell-Titer-Glo reagent. The dose-response curves are plotted with nonlinear regression analysis of GraphPad Prism.|
|Incubation time||1 h|
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
Metabolic stress boosts humoral responses in vivo independently of inflammasome and inflammatory reaction.
Andris F, et al. J Immunol. 2011 Feb 15;186(4):2245-53. PMID: 21248260.
Deficit of mitochondria-derived ATP during oxidative stress impairs mouse MII oocyte spindles.
Zhang X, et al. Cell Res. 2006 Oct;16(10):841-50. PMID: 16983401.
Mitochondria in eosinophils: functional role in apoptosis but not respiration.
Peachman KK, et al. Proc Natl Acad Sci U S A. 2001 Feb 13;98(4):1717-22. PMID: 11172017.
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