Nifedipine (BAY-a-1040), a potent vasodilator agent with calcium antagonistic action. It is a useful anti-anginal agent that also lowers blood pressure. Nifedipine (BAY-a-1040) is a dihydropyridine calcium channel blocker. Its main uses are as an antianginal (especially in Prinzmetal’s angina) and antihypertensive, although a large number of other uses have recently been found for this agent, such as Raynaud’s phenomenon, premature labor, and painful spasms of the esophagus in cancer and tetanus patients. It is also commonly used for the small subset of pulmonary hypertension patients whose symptoms respond to calcium channel blockers.
Cell Experiment | |
---|---|
Cell lines | TM4 cells |
Preparation method | Effects of extracellular calcium ion and calcium channel blocker on Sertoli cell phagocytosis. TM4 cells were cultured for two hours and then treated with or without 10 µM nifedipine for additional 20 hours. Fluorescent carboxyl microspheres were then added to wells for further two hours as usual. Culture medium of some untreated wells was replaced by one mM EGTA containing culture medium (Ca; 0.1 mM) just before addition of microspheres. Cells were then evaluated for phagocytosis by flow cytometric analysis. Calcium concentration of untreated or one mM EGTA growth medium was one mM or 0.1 mM, respectively. |
Concentrations | 10 µM |
Incubation time | 20 hours |
Animal Experiment | |
---|---|
Animal models | |
Formulation | |
Dosages | |
Administration |
Molecular Weight | 346.33 |
Formula | C17H18N2O6 |
CAS Number | 21829-25-4 |
Form | Solid |
Solubility (25°C) | DMSO 59 mg/mL |
Storage | -20°C, protect from light, sealed |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[1] N Tokuda, et al. J Urol. Phagocytosis by the murine testicular TM4 Sertoli cell line in culture
Related Calcium Channel Products |
---|
ω-Agatoxin TK
ω-Agatoxin TK, a peptidyl toxin of the venom of Agelenopsis aperta, is a potent and selective P/Q type Ca2+ channel blocker. |
SNX-482
SNX-482, a peptidyl toxin of the spider Hysterocrates gigas, is a potent, high affinity, selective and voltage-dependent R-type CaV2.3 channel blocker with an IC50 of 30 nM. |
ω-Conotoxin MVIIC
ω-Conotoxin MVIIC is a N- and P/Q-type Ca2+ channel blocker, significantly suppresses the 11-keto-βboswellic acid-mediated inhibition of glutamate release. |
ω-Conotoxin GVIA
ω-Conotoxin GVIA is an inhibitor of the N-type Ca2+ channel. |
ω-Conotoxin CnVIIA
ω-Conotoxin CnVIIA, a 27 amino acid neuropeptide toxin, is a N-type calcium current blocker. |
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.