The effects of oxysterols on macrophage migration have been shown to be partially inhibited with EBI2 antagonist (NIBR189) indicating EBI2 mediated signalling. EBI2 antagonist NIBR189 inhibited the effects of 7α25HC on macrophage migration, that was exogenously applied to the lower chamber of the xCELLigence® migration transwell system. Importantly, conditioned media taken from astrocytes stimulated with LPS for 6 and 8 hours (LPS-Astro-Med) and supplemented with EBI2 antagonist (NIBR189) attenuated the observed macrophage migration.
| Cell Experiment | |
|---|---|
| Cell lines | RAW264.7 cell |
| Preparation method | The migration of cells through the electrode array from the upper to lower chambers is measured by an increase in impedance. The bottom chamber of the plate was filled with either astrocyte conditioned media or serum free media supplemented with 7α25HC (0.01 μM) with or without EBI2 antagonist NIBR189 (10 μM). Cell migration was recorded immediately after the plate was re-inserted and recorded for 2 hours. |
| Concentrations | 10 μM |
| Incubation time | 2 h |
| Animal Experiment | |
|---|---|
| Animal models | |
| Formulation | |
| Dosages | |
| Administration | |
| Molecular Weight | 429.31 |
| Formula | C21H21BrN2O3 |
| CAS Number | 1599432-08-2 |
| Solubility (25°C) | 47 mg/mL in DMSO |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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