Lomustine (CeeNU) is an alkylating agent of value against both hematologic malignancies and solid tumors. Lomustine is a highly lipophilic nitrosourea compound which undergoes hydrolysis in vivo to form reactive metabolites. These metabolites cause alkylation and cross-linking of DNA. Other biologic effects include inhibition of DNA synthesis and some cell cycle phase specificity. Nitrosureas generally lack cross-resistance with other alkylating agents.
Cell Experiment | |
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Cell lines | XP cells |
Preparation method | Growing cell lines routinely as monolayers in DMEM supplemented with 10% foetal calf serum, 25 mm HEPES, glutamine and penicillin/streptomycin. Carrying cytotoxicity studies out in HEPES-free medium in a 5% CO2 atmosphere. Plating 750-1000 cells/well in 96 well plates and after overnight incubation,treating them for 2 hours with or without 33 μM BG. Then adding temozolomide or CCNU for 1 hour in the same medium, the final DMSO concentration not exceeding 1%. Growing the cells for a further 7 days in fresh medium and assaying for protein content by the NCI sulphorhodamine assay; growth studies show that during the assay period cells are in log phase growth . For the repeat temozolomide dosing schedule, cells are given consecutive 24 hours treatments, with fresh medium each day. Carrying assays out at least in duplicate. |
Concentrations | 133 μM |
Incubation time | 1 hour |
Animal Experiment | |
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Animal models | Dogs |
Formulation | Saline |
Dosages | 90 mg/m2 |
Administration | Orally |
Molecular Weight | 233.7 |
Formula | C9H16ClN3O2 |
CAS Number | 13010-47-4 |
Form | Solid |
Solubility (25°C) | DMSO 40 mg/mL |
Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[2] Alaaeddin Alrohaibani, et al. Microangiopathy, lomustine, and karyomegalic mesangial cells
[3] Michael Weller, et al. How did lomustine become standard of care in recurrent glioblastoma?
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