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Gemcitabine Hydrochloride

Cat. No. M1716
Gemcitabine Hydrochloride Structure
Synonym:

Gemzar, LY-188011

Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
50mg USD 42  USD42 In stock
100mg USD 53  USD53 In stock
200mg USD 69  USD69 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

Gemcitabine is an antimetabolites, which inhibits DNA synthesis with an IC50 of 0.06 µM. Gemcitabine is a nucleoside analogue that is incorporated into replicating DNA, resulting in partial chain termination and stalling of replication forks.

Product Citations
Protocol
Cell Experiment
Cell lines BxPC-3, PANC-1 and PaCa-2 cell lines
Preparation method Cell Counts. The cells were plated in 6-well plates. Twenty-four hours later, the cells were treated with vehicle, gemcitabine, DMAPT, or the combination. After 72 h of treatment, trypan blue-excluded cell counts were performed in duplicate using a hemocytometer. Cell growth was expressed relative to vehicle-treated cells (100 %).
Concentrations 0, 0.005, 0.01, 0.02, 0.04 μM
Incubation time 72 h
Animal Experiment
Animal models MIA PaCa-2 Xenografts model in athymic nude mice
Formulation phosphate-buffered saline, PBS
Dosages a single dose of 50 or 100 mg/kg
Administration intraperitoneal injection
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight 299.66
Formula C9H11F2N3O4.HCI
CAS Number 122111-03-9
Purity >99%
Solubility Water 50 mg/mL
DMSO 30 mg/mL
Storage 2-8°C, protect from light, dry, sealed
References

[1] Rich et al. Onco Targets Ther. Weekly paclitaxel, gemcitabine, and external irradiation followed by randomized farnesyl transferase inhibitor R115777 for locally advanced pancreatic cancer.

[2] Avan et al. Curr Pharm Des. Enhancement Of The Antiproliferative Activity Of Gemcitabine By Modulation Of C-Met Pathway In Pancreatic Cancer.

[3] Tajima et al. Exp Ther Med. Pilot study of neoadjuvant chemotherapy with gemcitabine and oral S-1 for resectable pancreatic cancer.

[4] Kasuya et al. Exp Ther Med. Combination therapy of gemcitabine or oral S-1 with the anti-VEGF monoclonal antibody bevacizumab for pancreatic neuroendocrine carcinoma.

[5] Holcomb BK, et al. J Gastrointest Surg. Dimethylamino parthenolide enhances the inhibitory effects of gemcitabine in human pancreatic cancer cells.

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Keywords: Gemcitabine Hydrochloride, Gemzar, LY-188011 supplier, Checkpoint, inhibitors

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