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Gemcitabine Hydrochloride

Cat. No. M1716
Gemcitabine Hydrochloride Structure
Synonym:

Gemzar, LY-188011

Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
50mg USD 42  USD42 In stock
100mg USD 53  USD53 In stock
200mg USD 69  USD69 In stock
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Quality Control & Documentation
Biological Activity

Gemcitabine is an antimetabolites, which inhibits DNA synthesis with an IC50 of 0.06 µM. Gemcitabine is a nucleoside analogue that is incorporated into replicating DNA, resulting in partial chain termination and stalling of replication forks.

Product Citations
Protocol (for reference only)
Cell Experiment
Cell lines BxPC-3, PANC-1 and PaCa-2 cell lines
Preparation method Cell Counts. The cells were plated in 6-well plates. Twenty-four hours later, the cells were treated with vehicle, gemcitabine, DMAPT, or the combination. After 72 h of treatment, trypan blue-excluded cell counts were performed in duplicate using a hemocytometer. Cell growth was expressed relative to vehicle-treated cells (100 %).
Concentrations 0, 0.005, 0.01, 0.02, 0.04 μM
Incubation time 72 h
Animal Experiment
Animal models MIA PaCa-2 Xenografts model in athymic nude mice
Formulation phosphate-buffered saline, PBS
Dosages a single dose of 50 or 100 mg/kg
Administration intraperitoneal injection
Chemical Information
Molecular Weight 299.66
Formula C9H11F2N3O4.HCI
CAS Number 122111-03-9
Solubility (25°C) Water 50 mg/mL
DMSO 30 mg/mL
Storage 2-8°C, protect from light, dry, sealed
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Rich et al. Onco Targets Ther. Weekly paclitaxel, gemcitabine, and external irradiation followed by randomized farnesyl transferase inhibitor R115777 for locally advanced pancreatic cancer.

[2] Avan et al. Curr Pharm Des. Enhancement Of The Antiproliferative Activity Of Gemcitabine By Modulation Of C-Met Pathway In Pancreatic Cancer.

[3] Tajima et al. Exp Ther Med. Pilot study of neoadjuvant chemotherapy with gemcitabine and oral S-1 for resectable pancreatic cancer.

[4] Kasuya et al. Exp Ther Med. Combination therapy of gemcitabine or oral S-1 with the anti-VEGF monoclonal antibody bevacizumab for pancreatic neuroendocrine carcinoma.

[5] Holcomb BK, et al. J Gastrointest Surg. Dimethylamino parthenolide enhances the inhibitory effects of gemcitabine in human pancreatic cancer cells.

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Keywords: Gemcitabine Hydrochloride, Gemzar, LY-188011 supplier, DNA/RNA Synthesis, inhibitors, activators


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