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Enalaprilat Dihydrate

Cat. No. M3426
Enalaprilat Dihydrate Structure
Size Price Availability Quantity
50mg USD 80 In stock
1g USD 400 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

Enalaprilat dihydrate is the first dicarboxylate-containing ACE inhibitor with an IC50 of 4 nM. It was developed partly to overcome these limitations of captopril. The sulfhydryl-moiety was replaced by a carboxylate-moiety, but additional modifications were required in its structure-based design to achieve a similar potency to captopril.

Protocol
Cell Experiment
Cell lines Rat cardiac fibroblasts cell lines
Preparation method After 24 hours incubation in serum-free medium (DMEM), stimulating cells with IGF-I (1-100 nM) and coincubating with Enalaprilat (1 nM-10 μM) for 24 hours. Using a colorimetric immunoassay during the last 4 hours of the 24 hours incubation period to assesse cellular proliferation by 5-bromo-2'-deoxyuridine (BrdU) incorporation . Mearsuring the extinctions at 450 nm in an ELISA plate reader. All values consist of an n=9.
Concentrations Dissolved in DMSO, final concentrations 1 nM - 10 μM
Incubation time 24 hours
Animal Experiment
Animal models Male Sprague–Dawley rats
Formulation Dissolved in a vehicle solution (1 mg in 950 μL of phosphate buffered saline and 50 μL 1M Na2CO3).
Dosages 1 mg/kg
Administration I.V. bolus
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 348.4
Formula C18H24N2O5.2H2O
CAS Number 84680-54-6
Purity >99%
Solubility DMSO 60 mg/mL
Storage at -20°C
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Quinapril hydrochloride

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Enalaprilat Dihydrate supplier, ACE, inhibitors

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