Cycloheximide is an inhibitor of protein biosynthesis in eukaryotic organisms, produced by the bacterium Streptomyces griseus. Cycloheximide exerts its effect by interfering with the translocation step in protein synthesis (movement of two tRNA molecules and mRNA in relation to the ribosome) thus blocking translational elongation. Cycloheximide is widely used in biomedical research to inhibit protein synthesis in eukaryotic cells studied in vitro (i.e. outside of organisms). It is inexpensive and works rapidly. Its effects are rapidly reversed by simply removing it from the culture medium.
Adv Sci (Weinh). 2024 Jan;e2207435.
Defective Homologous Recombination Repair By Up-Regulating Lnc-HZ10/Ahr Loop in Human Trophoblast Cells Induced Miscarriage
Cycloheximide purchased from AbMole
Mol Carcinog. 2024 Jan.
SN-38, an active metabolite of irinotecan, inhibits transcription of nuclear factor erythroid 2-related factor 2 and enhances drug sensitivity of colorectal cancer cells
Cycloheximide purchased from AbMole
iScience. 2024 Jan 19.
FLOT2 promotes nasopharyngeal carcinoma progression through suppression of TGF-β pathway via facilitating CD109 expression
Cycloheximide purchased from AbMole
Mol Cell Biochem. 2024 Feb 17.
Tripartite-motif 3 represses ovarian cancer progression by downregulating lactate dehydrogenase A and inhibiting AKT signaling
Cycloheximide purchased from AbMole
J Transl Med. 2023 May 5;21(1):303.
SYTL2 promotes metastasis of prostate cancer cells by enhancing FSCN1-mediated pseudopodia formation and invasion
Cycloheximide purchased from AbMole
Cell Death Discov. 2023 Apr 26.
Ammonium Tetrathiomolybdate Relieves Oxidative Stress in Cisplatin-induced Acute Kidney Injury via NRF2 Signaling Pathway
Cycloheximide purchased from AbMole
Cell Death Discov. 2023 Jul 25;9(1):259.
Ammonium tetrathiomolybdate relieves oxidative stress in cisplatin-induced acute kidney injury via NRF2 signaling pathway
Cycloheximide purchased from AbMole
J Cell Mol Med. 2023 Aug 29.
Alleviation of temporomandibular joint osteoarthritis by targeting RIPK1-mediated inflammatory signalling
Cycloheximide purchased from AbMole
FASEB J. 2023 Oct;37(10):e23183.
IGF2BP2 promotes ovarian cancer growth and metastasis by upregulating CKAP2L protein expression in an m6A-dependent manner
Cycloheximide purchased from AbMole
New Journal of Chemistry. 2023 Jun 20.
Quantitative analysis of the effects of vimentin intermediate filaments on the early stages of influenza A virus infection
Cycloheximide purchased from AbMole
New J. Chem. 2023 Jun 28;47, 14344-14354.
Quantitative analysis of the effects of vimentin intermediate filaments on the early stages of influenza A virus infection
Cycloheximide purchased from AbMole
Biochem Genet. 2023 Nov 11.
Functional Characterization of a Novel SLC4A4 Variant and Uniparental Isodisomy in Proximal Renal Tubular Acidosis Patient
Cycloheximide purchased from AbMole
Cell Death Differ. 2022 Jul;29(7):1395-1408.
SARS-CoV-2 membrane protein causes the mitochondrial apoptosis and pulmonary edema via targeting BOK
Cycloheximide purchased from AbMole
Research Square. 2022 Oct 12.
IGF2BP2 promotes ovarian cancer growth and metastasis via upregulating CKAP2L protein expression in a m6A-dependent manner
Cycloheximide purchased from AbMole
Ecotoxicol Environ Saf. 2022 Jun 1;237:113564.
Environmental BPDE induced human trophoblast cell apoptosis by up-regulating lnc-HZ01/p53 positive feedback loop
Cycloheximide purchased from AbMole
Virol Sin. 2022 Aug 16;S1995-820X(22)00143-2.
CK1α upregulates the IFNAR1 expression to prompt the anti-HBV effect of type I IFN in hepatoma carcinoma cells
Cycloheximide purchased from AbMole
Cell Biol Toxicol. 2022 Apr;38(2):291-310.
Lnc-HZ08 regulates BPDE-induced trophoblast cell dysfunctions by promoting PI3K ubiquitin degradation and is associated with miscarriage
Cycloheximide purchased from AbMole
Bioengineered. 2022 Apr;13(4):8000-8012.
UBE2B promotes ovarian cancer growth via promoting RAD18 mediated ZMYM2 monoubiquitination and stabilization
Cycloheximide purchased from AbMole
J Biol Chem. 2022 Nov 21;102734.
Ubiquitin-specific peptidase 14 maintains estrogen receptor α stability via its deubiquitination activity in endometrial cancer
Cycloheximide purchased from AbMole
Mol Cell Endocrinol. 2022 Apr 5;545:111575.
An intron mutation of HNF1A causes abnormal splicing and impairs its activity as a transcription factor
Cycloheximide purchased from AbMole
Adv Sci (Weinh). 2021 Feb.
Inhibition Lysosomal Degradation of Clusterin by Protein Kinase D3 Promotes Triple‐Negative Breast Cancer Tumor Growth
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Adv Sci (Weinh). 2021 Jan 6;8(4):2003205.
Inhibition Lysosomal Degradation of Clusterin by Protein Kinase D3 Promotes Triple-Negative Breast Cancer Tumor Growth
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Clin Transl Med. 2021 Apr;11(4):e395.
FABP4 deactivates NF-κB-IL1α pathway by ubiquitinating ATPB in tumor-associated macrophages and promotes neuroblastoma progression
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Int J Mol Sci. 2021 Aug 16;22(16):8779.
Autophagy Mediates the Degradation of Plant ESCRT Component FREE1 in Response to Iron Deficiency
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J Cancer. 2021 Oct 17;12(23):7041-7051.
N6-methyladenosine regulates ATM expression and downstream signaling
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Cell Rep. 2020 Jul 28;32(4):107974.
L ARP7 Is a BRCA1 Ubiquitinase Substrate and Regulates Genome Stability and Tumorigenesis
Cycloheximide purchased from AbMole
Cell Rep. 2020 Jul 28;32(4):107974.
LARP7 is a BRCA1 ubiquitinase substrate and regulates genome stability and tumorigenesis
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Theranostics. 2020 Apr 27;10(13):5671-5686.
SUMO1 modification of methyltransferase-like 3 promotes tumor progression via regulating Snail mRNA homeostasis in hepatocellular carcinoma
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Cell Oncol (Dordr). 2020 Oct 19.
Ubiquitin-specific protease 2a promotes hepatocellular carcinoma progression via deubiquitination and stabilization of RAB1A
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Cell Microbiol. 2020 Mar;22(3):e13148.
Hepatitis B virus X protein modulates upregulation of DHX9 to promote viral DNA replication.
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Life Sci. 2020 Sep 15;257:118021.
TRIB3 destabilizes tumor suppressor PPARα expression through ubiquitin-mediated proteasome degradation in acute myeloid leukemia
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Front Cell Infect Microbiol. 2020 Apr 16;10:158.
Difluoromethylornithine, a Decarboxylase 1 Inhibitor, Suppresses Hepatitis B Virus Replication by Reducing HBc Protein Levels
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Aging (Albany NY). 2018 Oct 18;10(10):2783-2799.
Ubiquitin-specific protease 4 promotes metastasis of hepatocellular carcinoma by increasing TGF-β signaling-induced epithelial-mesenchymal transition.
Cycloheximide purchased from AbMole
Biotechnol Lett. 2018 Aug;40:1181-1188.
UHRF2 promotes DNA damage response by decreasing p21 via RING finger domain.
Cycloheximide purchased from AbMole
Protein Cell. 2017 Mar;8(3):202-218.
E3 ligase UHRF2 stabilizes the acetyltransferase TIP60 and regulates H3K9ac and H3K14ac via RING finger domain
Cycloheximide purchased from AbMole
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Source | Biotechnol Lett (2018 Aug). Figure 3. cycloheximide (Abmole Bioscience) |
| Method | Western blotting | |
| Cell Lines | Human embryonic kidney 293 cells | |
| Concentrations | 50 ug/mL | |
| Incubation Time | 3 h | |
| Results | The overexpression of UHRF2 shortened the half-life of p21 to 1 h compared to 3 h under controlled conditions. |
. figure 4.jpg) |
Source | Protein and Cell (2016). Figure 4.CHX (ABmole Bioscience, USA) |
| Method | Half-life experiments | |
| Cell Lines | Human normal liver cells (LO2) | |
| Concentrations | 100 μg/mL | |
| Incubation Time | ||
| Results | Graph represents collated results rom three independent half-life experiments performed in HEK293, LO2 and HepG2 cells. TIP60 levels were normalized to 1 at time zero. |
| Molecular Weight | 281.35 |
| Formula | C15H23NO4 |
| CAS Number | 66-81-9 |
| Solubility (25°C) | DMSO ≥ 80 mg/mL Water 15 mg/mL (ultrasonic and warming) |
| Storage | 4°C, protect from light |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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Alternariol monomethyl ether is an alternaria mycotoxin and genotoxin, found in common edible crops. It inhibits the activity of various DNA-topoisomerases, increasing the rate of DNA strand breaks. |
| APE1-IN-1
APE1-IN-1 is a potent and blood-brain barrier (BBB) penetrant apurinic/apyrimidinic (AP) endonuclease 1 (APE1) inhibitor with an IC50 value of 2 μM. APE1-IN-1 can potentiate the cytotoxicity of the alkylating agents Methylmethane sulfonate to cancer cells. |
| DTP3 TFA
DTP3 TFA is a potent and selective GADD45β/MKK7 (growth arrest and DNA-damage-inducible β/mitogen-activated protein kinase kinase 7) inhibitor. |
| Mating Factor α (1-6)
Mating Factor α (1-6) is a mating factor produced by α-mating type cells of Saccharomyces cerevisiae and an acts as an inhibitor of the initiation of DNA synthesis in the cells. |
| Polynucleotide kinase
Polynucleotide Kinase is a DNA repair enzyme. |
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