Cilastatin attenuates VAN-induced acute renal failure in vitro by decreasing apoptosis without affecting antibacterial activity. This effect could be related, at least in part, to the reduction in accumulation of the drug in cells. Therefore, cilastatin could represent a novel therapeutic approach in reducing VAN-induced renal damage without compromising bactericidal efficacy.
|Cell lines||Proximal Tubular Primary Cell|
|Preparation method||Cells were pretreated with VAN (0.6, 3, and 6 mg/mL) alone or in combination with cilastatin (200 μg/mL) before being trypsinized, washed twice with PBS, and incubated for 30 min in the dark in 100 μL buffer containing 5 μL fluorescein isothiocyanate- (FITC-) labeled annexin V and 5 μL PI for flow cytometry.|
|Incubation time||24 h|
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
|Solubility||10 mM in DMSO|
Protective Effects of Cilastatin against Vancomycin-Induced Nephrotoxicity.
Humanes B, et al. Biomed Res Int. 2015;2015:704382. PMID: 26504822.
Randomized phase 2 trial to evaluate the clinical efficacy of two high-dosage tigecycline regimens versus imipenem-cilastatin for treatment of hospital-acquired pneumonia.
Ramirez J, et al. Antimicrob Agents Chemother. 2013 Apr;57(4):1756-62. PMID: 23357775.
6-Chloropurine is used in the preparation of 9-alkylpurines through alkylation with various substituted alkyl halides in dimethyl sulfoxide.
Lanolin is a wax secreted by the sebaceous glands of wool-bearing animals and used widely in the pharmaceutical and cosmetics industries.
Potassium acetate is the potassium salt of acetic acid, which is a synthetic carboxylic acid with antibacterial and antifungal properties.
5-Phenyl-2,4-pentadienoic acid is used as Synthetic building block, anti-malarial agent.
Methyl 4-hydroxybenzoate is an anti-fungal agent often used in a variety of cosmetics, personal-care products and food preservatives.
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2017 AbMole BioScience. All Rights Reserved.