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Cidofovir

Cat. No. M1695
Cidofovir Structure
Synonym:

Vistide; GS 0504; HPMPC

Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
5mg USD 23.4  USD26 In stock
10mg USD 42.3  USD47 In stock
50mg USD 81  USD90 In stock
100mg USD 131.4  USD146 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

Cidofovir (Vistide) is an injectable antiviral medication for the treatment of cytomegalovirus (CMV) retinitis (IC50 = 0.94 μM). Cidofovir suppresses CMV replication by selective inhibition of viral DNA polymerase and therefore prevention of viral replication and transcription.

Product Citations
Customer Product Validations & Biological Datas
Source Towson University thesis(2015) . Figure 12. cidofovir (Abmole, Harbour City, Kowloon Hong Kong)
Method Plaque Assay
Cell Lines Equine dermal fibroblast (E. Derm) cells
Concentrations 7.5 µg/mL
Incubation Time 4 d
Results Well plates pre-treated with 7.5 µg/mL CDV contained some uninfected cells.
Source Towson University thesis(2015) . Figure 7 .cidofovir (Abmole, Harbour City, Kowloon Hong Kong)
Method MTT assay
Cell Lines Equine dermal fibroblast (E. Derm) cells
Concentrations 1, 2.5, 5, 7.5, and 10 µg/mL
Incubation Time 24 h
Results The combination of CDV and ACV at these chosen concentrations was found to be non-toxic for the cells since the lowest observed cell viability was around 78%.
Source Towson University thesis(2015) . Figure 6 .cidofovir (Abmole, Harbour City, Kowloon Hong Kong)
Method MTT assay
Cell Lines Equine dermal fibroblast (E. Derm) cells
Concentrations 1, 5, 12.5, and 17.5 µg/mL
Incubation Time 24 h
Results "CDV combined with FOS at these chosen concentrations were found to be non-toxic for the cells since the lowest observed cell viability was around 85%."
Source Towson University thesis(2015) . Figure 4 .cidofovir (Abmole, Harbour City, Kowloon Hong Kong)
Method MTT assay
Cell Lines Equine dermal fibroblast (E. Derm) cells
Concentrations 1, 5, 10, 15, 20, 25, and 30 µg/mL
Incubation Time 24 h
Results CDV’s CC50 value was around 27.5 µg/mL.
Protocol
Cell Experiment
Cell lines Madin–Darby Canine Kidney or Vero cells
Preparation method In vitro efficacy of cidofovir against CHV-1 Madin–Darby Canine Kidney or Vero cells were plated into 96-well plates at 1*104 cells/well and maintained in Dulbecco’s minimal essential medium with 1 g/L glucose, l-glutamine, and sodium pyruvate (Cell Grow; Corning, Manassas, VA) containing 10% fetal bovine serum (Atlanta Biological, Flowery Branch, GA) and penicillin (200 U/mL)/ streptomycin (200 mg/mL) (Life Technologies, Grand Island, NY) for 24 h until confluent. Monolayers were infected with 5 PFU/well CHV-1 (a previously described field strain that was also utilized during the in vivo experimental study evaluating cidofovir) or HSV-1 strain F, respectively. After 2 h of incubation at 37 C, virus supernatants were removed, cells were rinsed once with phosphate-buffered saline (PBS), and 100 mL of sequential 2-fold serial dilutions of cidofovir (starting at 625 mM) was added to each well. Infection controls without cidofovir and noninfected drug-treated controls were also included. Plates were incubated at 37 C until a cytopathic effect (CPE) was visible in the infected nontreated controls, which was *72 hours postinfection (hpi) for CHV-1 and 48 hpi for HSV-1, respectively. Plates were rinsed 3 times with PBS, fixed in 80% ethanol for 10 min at -20 C, and stained with crystal violet (Fisher Scientific, Fair Lawn, NJ). Virusinduced CPE was scored as the percent of wells per dilution with observable plaque formation, and EC50 values were calculated using GraphPad Prism.
Concentrations 0~625µM
Incubation time 72 hours postinfection (hpi) for CHV-1 and 48 hpi for HSV-1
Animal Experiment
Animal models Recurrent ocular CHV-1 infection in dogs by administration of systemic prednisolone (3.0 mg/kg PO q24 h) for 7 consecutive days beginning on study day 1.
Formulation 0.9% sodium chloride solution
Dosages 1 drop of 0.5% cidofovir ophthalmic solution
Administration eyes drop
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight 279.19
Formula C8H14N3O6P
CAS Number 113852-37-2
Purity >99%
Solubility DMSO
Storage at -20°C
References

Successful Response to Cidofovir of Adenovirus Hepatitis During Chemotherapy in a Child With Hepatoblastoma.
Mateos et al. J Pediatr Hematol Oncol. 2012 Aug 29. PMID: 22935664.

The impact of cidofovir treatment on viral loads in adult recurrent respiratory papillomatosis.
Mikolajczak et al. Eur Arch Otorhinolaryngol. 2012 Jul 6. PMID: 22766836.

A single cidofovir treatment rescues animals at progressive stages of lethal orthopoxvirus disease.
Israely et al. Virol J. 2012 Jun 18;9:119. PMID: 22709563.

Successful clearance of cutaneous acyclovir-resistant, foscarnet-refractory herpes virus lesions with topical cidofovir in an allogeneic hematopoietic stem cell transplant patient.
Muluneh et al. J Oncol Pharm Pract. 2012 May 30. PMID: 22635417.

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Cidofovir, Vistide; GS 0504; HPMPC supplier, Anti-infection, inhibitors

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