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Calcium N5-methyltetrahydrofolate

Cat. No. M6109
Calcium N5-methyltetrahydrofolate Structure
Synonym:

NSC173328

Size Price Availability Quantity
5mg USD 350 In stock
10mg USD 500 In stock
50mg USD 1500 In stock
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Quality Control
  • Current batch:
  • Purity >99%
  • COA
  • MSDS
Biological Activity

The results of the current study demonstrate that the HRPE cells express the reduced-folate transporter that mediates the cellular uptake of N5 methyltetrahydrofolate, the predominant form of folate in blood.

Protocol
Cell Experiment
Cell lines HRPE cells
Preparation method One milliliter of a hypotonic medium (10 mM Tris-HCl buffer, pH 8) containing 1 μM unlabeled N5-methyltetrahydrofolate was added to each well, and die cells were lysed by freezing and thawing.
Concentrations 1 μM
Incubation time 2 h
Animal Experiment
Animal models
Formulation
Dosages
Administration
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 497.52
Formula C20H23CaN7O6
CAS Number 26560-38-3
Purity >99%
Solubility 10 mM in DMSO
Storage at -20°C
References

Methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase (MeTr): protonation state of the ligand and active-site residues.
Alonso H, et al. J Phys Chem B. 2009 Nov 5;113(44):14787-96. PMID: 19827815.

Characterization of N5-methyltetrahydrofolate uptake in cultured human retinal pigment epithelial cells.
Huang W, et al. Invest Ophthalmol Vis Sci. 1997 Jul;38(8):1578-87. PMID: 9224286.

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Keywords: Calcium N5-methyltetrahydrofolate, NSC173328 supplier, inhibitors

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