The results of the current study demonstrate that the HRPE cells express the reduced-folate transporter that mediates the cellular uptake of N5 methyltetrahydrofolate, the predominant form of folate in blood.
|Cell lines||HRPE cells|
|Preparation method||One milliliter of a hypotonic medium (10 mM Tris-HCl buffer, pH 8) containing 1 μM unlabeled N5-methyltetrahydrofolate was added to each well, and die cells were lysed by freezing and thawing.|
|Incubation time||2 h|
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
|Solubility||10 mM in DMSO|
Methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase (MeTr): protonation state of the ligand and active-site residues.
Alonso H, et al. J Phys Chem B. 2009 Nov 5;113(44):14787-96. PMID: 19827815.
Characterization of N5-methyltetrahydrofolate uptake in cultured human retinal pigment epithelial cells.
Huang W, et al. Invest Ophthalmol Vis Sci. 1997 Jul;38(8):1578-87. PMID: 9224286.
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