Blasticidin S hydrochloride

Biological Activity

Blasticidin S HCl is a potent inhibitor of protein synthesis in both prokaryotic and eukaryotic cells. Blasticidin S Hydrochloride belongs to a group of cytosine amino nucleoside antibiotics. It has a wide spectrum of antimicrobial activity and acts on prokaryotic and eukaryotic organisms by inhibiting protein synthesis.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information

References

Critical role of the proton-dependent oligopeptide transporter (POT) in the cellular uptake of the peptidyl nucleoside antibiotic, blasticidin S.
Kitamura K, et al. Biochim Biophys Acta Mol Cell Res. 2017 Feb;1864(2):393-398. PMID: 27916534.

Crystal and molecular structure of the antibiotic blasticidin S hydrochloride pentahydrate.
Swaminathan V, et al. Biochim Biophys Acta. 1981 Oct 27;655(3):335-41. PMID: 7284390.