Actinomycin D is a compound composed of a two CYCLIC PEPTIDES attached to a phenoxazine that is derived from STREPTOMYCES parvullus. It binds to DNA and inhibits RNA synthesis (transcription), with chain elongation more sensitive than initiation, termination, or release. As a result of impaired mRNA production, protein synthesis also declines after dactinomycin therapy.
Theranostics. 2020 Apr 27;10(13):5671-5686.
SUMO1 modification of methyltransferase-like 3 promotes tumor progression via regulating Snail mRNA homeostasis in hepatocellular carcinoma
Actinomycin D purchased from AbMole
|Source||Theranostics (2020 Apr). Figure 5. Actinomycin D (Abmole Bioscience, US)|
|Method||RNA decay assay|
|Cell Lines||MHCC97H and HepG2 cells|
|Results||We next sought to assess the RNA decay rate in Mettl3-WT and Mettl3-KR cells treated with the transcription inhibitor actinomycin D to block new RNA synthesis. Snail mRNA expression was decreased, and the Snail mRNA half-lives were significantly shortened in SUMO-mutant Mettl3 cells|
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
|Solubility||DMSO ≥ 30 mg/mL|
JNJ-26481585 primes rhabdomyosarcoma cells for chemotherapeutics by engaging the mitochondrial pathway of apoptosis.
Heinicke U,et.al. Oncotarget. 2015 Oct 12. PMID: 26473375.
Il-19 reduces VSMC activation by regulation of mRNA regulatory factor HuR and reduction of mRNA stability
Anthony A Cuneo, et al. J Mol Cell Cardiol. 2010 Oct;49(4):647-54. PMID: 20451530.
Stability of mRNA in the hyperthermophilic archaeon Sulfolobus solfataricus
Elisabetta Bini, et al. RNA. 2002 Sep;8(9):1129-36. PMID: 12358432.
Superinduction of IL-2 gene expression by vomitoxin (deoxynivalenol) involves increased mRNA stability
S Li, et al. Toxicol Appl Pharmacol. 1997 Dec;147(2):331-42. PMID: 9439728.
|Related DNA/RNA Synthesis Products|
Beaucage reagent is a potent DNA cleavage agent. Beaucage reagent can be used for preparing phosphorothioate oligomers.
L189 is a novel human DNA ligase inhibitor with IC50s of 5 μM, 9 μM and 5 μM for hLigI, hLigIII and hLigIV, respectively.
Saccharin 1-methylimidazole (also known as SMI) is an activator for DNA and RNA synthesis.
Lurbinectedin, also known as PM01183, is a covalent DNA minor groove binder with potential antineoplastic activity.
|Bleomycin A5 Hydrochloride
Bleomycin A5 Hydrochloride is a unique antibiotic of the bleomycin family that is toxic to eukaryotic and prokaryotic cells.
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