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Actinomycin D

Cat. No. M4881
Actinomycin D Structure

Actinomycin Aiv; Dactinomycin; Act D; RASP-101

Size Price Availability Quantity
5mg USD 49.5  USD55 In stock
10mg USD 85.5  USD95 In stock
50mg USD 360  USD400 In stock
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Quality Control
Biological Activity

Actinomycin D is a compound composed of a two CYCLIC PEPTIDES attached to a phenoxazine that is derived from STREPTOMYCES parvullus. It binds to DNA and inhibits RNA synthesis (transcription), with chain elongation more sensitive than initiation, termination, or release. As a result of impaired mRNA production, protein synthesis also declines after dactinomycin therapy.

Product Citations
Customer Product Validations & Biological Datas
Source Theranostics (2020 Apr). Figure 5. Actinomycin D (Abmole Bioscience, US)
Method RNA decay assay
Cell Lines MHCC97H and HepG2 cells
Concentrations 5 μg/mL
Incubation Time -
Results We next sought to assess the RNA decay rate in Mettl3-WT and Mettl3-KR cells treated with the transcription inhibitor actinomycin D to block new RNA synthesis. Snail mRNA expression was decreased, and the Snail mRNA half-lives were significantly shortened in SUMO-mutant Mettl3 cells
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight 1255.41
Formula C62H86N12O16
CAS Number 50-76-0
Purity 99.73%
Solubility DMSO ≥ 30 mg/mL
Storage at -20°C

JNJ-26481585 primes rhabdomyosarcoma cells for chemotherapeutics by engaging the mitochondrial pathway of apoptosis.
Heinicke U, Oncotarget. 2015 Oct 12. PMID: 26473375.

Il-19 reduces VSMC activation by regulation of mRNA regulatory factor HuR and reduction of mRNA stability
Anthony A Cuneo, et al. J Mol Cell Cardiol. 2010 Oct;49(4):647-54. PMID: 20451530.

Stability of mRNA in the hyperthermophilic archaeon Sulfolobus solfataricus
Elisabetta Bini, et al. RNA. 2002 Sep;8(9):1129-36. PMID: 12358432.

Superinduction of IL-2 gene expression by vomitoxin (deoxynivalenol) involves increased mRNA stability
S Li, et al. Toxicol Appl Pharmacol. 1997 Dec;147(2):331-42. PMID: 9439728.

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Keywords: Actinomycin D, Actinomycin Aiv; Dactinomycin; Act D; RASP-101 supplier, DNA/RNA Synthesis, inhibitors

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