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Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO)

Cat. No. M5293

Size Price Availability Quantity
1 mL USD 37 In stock
1 mL x 10 USD 270 In stock
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AbMole Protease Inhibitor Cocktail is a protease inhibitor composed of 6 independent protease inhibitors (excluding EDTA), which are configured according to the optimum mixture ratio. The target is respectively serine proteinase, serine cysteine proteinase, aminopeptidase, cysteine proteinase and aspartic proteinase, which are widely used in various proteases and can be called broad spectrum protease inhibitors.


Functional principles:

Protease inhibitors effectively improve protein production by inhibiting protease activity and reducing proteolytic breakdown of proteases, whichdoes not change the nature of cells and tissues themselves.


Handling introduction:

1. This product is suitable for the extraction and purification of proteins from mammalian cells and tissues, and Western blotting (WesternBlot),  Immunoprecipitation (Co-IP), immunofluorescence (IF), immunohistochemistry (IHC), kinase assay, antibodies and enzyme diagnostic kits (Dignose, Kit) etc.

2. In accordance with the volume ratio of 1:100, Cocktail is added in advance to the prepared experimental system and mix it gently.


Announcements:

1. This product does not contain EDTA, it is a 100 * DMSO storage fluid form. The concentration of cocktail should be adjusted to 1 * when you use it. If the cells or tissues are rich in protease, you may increase the concentration of cocktail in the experimental system.

2. When stored at -20 degrees centigrade, DMSO appears ice crystal . This is a normal phenomenon, not quality problems.

3. This product is for scientific research only.


FAQ:

1. What are the advantages of the protease inhibitor Cocktail?

Answers: Aiming at the target of many amino acids, the target protein is protected from the endogenous protease. The rate of protein extraction is higher, which greatly improves the experimental efficiency.


2. Can it be used in combination with cell lysate?

Answer: Yes, this can fully split cells or tissue proteins, effectively reducing protein degradation.


3. Why do I need to dissolve with DMSO? Can I exchange it for other solvents?

Answers: Protease Inhibitor Cocktail is easier to dissolve in DMSO, and DMSO usually does not affect the experimental results. It is always stable and prone to preserve and transport, therefore, other solvents are not taken into consideration for the time being.

 

4. When placed at room temperature mistakenly, will it be deteriorated?

Answers: It is possible to place it at room temperature for a short period of time without causing deterioration because protease inhibitors are usually relatively stable. If the experiment needs to be done at room temperature, it is recommended to be placed on ice or at a temperature of -20 degrees centigrade. And should avoid freezing and thawing repeatedly at the same time.


Documentation Download

Protease Inhibitor Cocktail (EDTA-Free, 100X in DMSO) Manual

Customer Product Validation
Source FEMS Microbiol Lett (2010). Figure 1. Protease Inhibitor Cocktail
Method Assay for proteomic analysis
Cell Lines Saliva samples
Concentrations 20 μL
Incubation Time 16 h
Results The correlation of bacterial growth (measured by CFU counts) on various media in the presence or absence of protease inhibitor cocktail was significant between the paired samples.
Rating
Source Korean J Lab Med. (2009). Figure 1. Protease Inhibitor Cocktail
Method PTH measurement
Cell Lines Blood samples
Concentrations 0.3 µM aprotinin, 130 µM bestatin, 1,000 µM EDTA, 14 µM E-64, 1 µM leupeptin, and 2,000 µM 4-(2-Aminoethyl mix
Incubation Time 48 h
Results All the samples except the samples with protease inhibitor cocktail showed significant reductions in PTH level during 48 hr storage (P<0.05). After 48 hr, the mean PTH levels in samples stored at room temperature and stored at 4℃ without protease inhibitors decreased by 40.7% and 20.1%, respectively.
Rating
Source Antimicrob Agents Chemother (2005). Figure 3. Protease Inhibitor Cocktail
Method Killing assays
Cell Lines C. albicans cells
Concentrations 1× PIC contains 2 mM 4-(2-aminoethyl
Incubation Time 1.5 h
Results Killing assays performed in clarified whole human saliva samples from five subjects, using the peptides at a concentration of 50 μM. From 60 to 100% killing of C. albicans was observed in saliva containing PIC, while only 6 to 60% was observed in saliva without PIC. The difference is statistically significant (P < 0.05).
Rating
Source Antimicrob Agents Chemother (2005). Figure 2. Protease Inhibitor Cocktail
Method Peptide stability
Cell Lines C. albicans cells
Concentrations 1× PIC contains 2 mM 4-(2-aminoethyl
Incubation Time 0.5 h
Results MUC7 12-mer-d was resistant to degradation by trypsin. The other three peptides, MUC7 12-mer-l, Hsn5 12-mer, and magainin-II, were degraded by trypsin. Similarly, MUC7 12-mer-d was resistant to degradation by unclarified saliva, and the other peptides were degraded by saliva, although to different degrees.
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Abmole Inhibitor Catalog 2017





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