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Rotenone

Cat. No. M6209
Rotenone Structure
Synonym:

NSC26258

Size Price Availability Quantity
Free Sample (0.5-1 mg)  USD 0 In stock
10mM/1mL In DMSO USD 120 In stock
1g USD 110 In stock
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Quality Control
Biological Activity

In vitro: Mitogen Activated Protein Kinase (MAPK), Toll-like receptor, Wnt, and Ras signaling pathways are intensively involved in the effect of rotenone on the ENS. Rotenone-induced cell death is reduced as measured by decline in the levels of pro-apoptotic proteins.

In vivo: Rotenone causes a significant increase in the excitatory amino acid neurotransmitters; glutamate and aspartate together with a significant decrease in the inhibitory amino acids, GABA, glycine and taurine are observed in the cerebellum of rat model of PD. Rotenone (1.5, 2, or 2.5 mg/kg) causes a dose-dependent increase in α-synuclein in the substantia nigra. Furthermore, at 2 and 2.5 mg/kg, rotenone causes a significant decrease in the number of tyrosine hydroxylase-immunoreactive neurons in the substantia nigra, and dopamine in the striatum in rats.

Protocol
Cell Experiment
Cell lines PC12 cells
Preparation method For cell or brain tissue exposure, rotenone stock solutions were diluted with PBS to obtain a final solution of 1m DMSO. PC12 cells were exposed to rotenone or the corresponding solvent for different times.
Concentrations 10 μM
Incubation time 48 h
Animal Experiment
Animal models adult male Sprague–Dawley rats
Formulation DMSO
Dosages 12 μg/rat
Administration i.v.
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 394.42
Formula C23H22O6
CAS Number 83-79-4
Purity >95%
Solubility 10 mM in DMSO
Storage at -20°C
References

Metabolic enhancer piracetam attenuates rotenone induced oxidative stress: a study in different rat brain regions.
Verma DK, et al. Acta Neurobiol Exp. 2015;75(4):399-411. PMID: 26994419.

The molecular mechanism of rotenone-induced α-synuclein aggregation: emphasizing the role of the calcium/GSK3β pathway.
Yuan YH, et al. Toxicol Lett. 2015 Mar 4;233(2):163-71. PMID: 25433145.

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Rotenone, NSC26258 supplier, inhibitors

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