Leptin is a cytokine belonging to the Interleukin 6 family, and has a four-helix bundle structure. Leptin also has effects on reproduction and immunity.
ED50 < 10.0 µg/ml, measured by a cell proliferation assay using LoVo cells, corresponding to a specific activity of > 100.0 units/mg.
Accession #: P50596
Apparent Molecular Weight: ~16.3 kDa, on SDS-PAGE under reducing conditions.
Endotoxin Level: < 0.2 EU/μg of protein by gel clotting method
Lyophilized after extensive dialysis against 50 mM Tris, pH 8.0.
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH2O up to 100 μg/ml. |
| Storage | Stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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