Expression system: CHO
Purity: >95% by SDS-PAGE analysis. > 95% by HPLC analysis
Endotoxin: < 0.2 EU/μg of protein by gel coagulation
Apparent molecular weight: 8~22 kDa on SDS-PAGE under reducing conditions
Storage and Stability: This product is stable for up to 6 months at temperatures below -70°C after receipt. After reconstitution, the product should be stable for 1 week at 4°C or 3 months at -20°C. For long-term storage, the addition of a carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles
Bioactivity: Interleukin-10 (IL-10), originally known as cytokine synthesis inhibitory factor (CSIF), belongs to the IL-10 family and shares more than 80% sequence homology with the Epstein-Barr virus protein BCRFI. It is produced by many immune cells, such as T cells, macrophages, mast cells and dendritic cells. It is usually secreted as a homodimer and, upon binding to receptors, inhibits the synthesis of several cytokines, including IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and Th2 cells. it also shows the ability to inhibit the function of antigen-presenting cells (APCs). The net effect of interleukin-10 appears to be inhibitory; however, stimulatory effects, such as stimulation of B-cell maturation and antibody production, have also been reported.
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
|Purity||>95% , Endotoxin < 0.2 EU/μg|
|Solubility||Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml.|
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