Recombinant Rat IL-10 (CHO)

Biological Activity

Genus: Rat
Expression system: CHO
Purity: >95% by SDS-PAGE analysis. > 95% by HPLC analysis
Endotoxin: < 0.2 EU/μg of protein by gel coagulation
Apparent molecular weight: 8~22 kDa on SDS-PAGE under reducing conditions
Storage and Stability: This product is stable for up to 6 months at temperatures below -70°C after receipt. After reconstitution, the product should be stable for 1 week at 4°C or 3 months at -20°C. For long-term storage, the addition of a carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles


Bioactivity: Interleukin-10 (IL-10), originally known as cytokine synthesis inhibitory factor (CSIF), belongs to the IL-10 family and shares more than 80% sequence homology with the Epstein-Barr virus protein BCRFI. It is produced by many immune cells, such as T cells, macrophages, mast cells and dendritic cells. It is usually secreted as a homodimer and, upon binding to receptors, inhibits the synthesis of several cytokines, including IFN-gamma, IL-2, IL-3, TNF, and GM-CSF produced by activated macrophages and Th2 cells. it also shows the ability to inhibit the function of antigen-presenting cells (APCs). The net effect of interleukin-10 appears to be inhibitory; however, stimulatory effects, such as stimulation of B-cell maturation and antibody production, have also been reported.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information