Midkine is a heparin-binding growth factor, which contains 121 amino acid residues with 5 disulfide bonds. Midkine promotes the growth, survival, and migration of various cells, and plays roles in neurogenesis and epithelial mesenchymal interactions during organogenesis. The C-terminal portion contains the heparin-binding domain and the antigenicity and neurite-promoting sequences. Both the C- and N-terminals are necessary for the molecule′s neurotrophic effects.
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-100 ng/ml.
Expression system : E. coli
Accession: P12025
Approximately Molecular Weight: 13.2 kDa, a single non-glycosylated polypeptide chain containing 120 amino acids.
AA Sequence:
VAKKKEKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRVHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK TKAKKGKGKD
Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH7.4.
Solubility (25°C) | Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. |
Storage | Stable for up to twelve months from date of receipt at -20℃ to -70℃. Reconstituted protein solution can be stored at 2-8°C for 2 weeks, at ≤ -20°C for 3 months. |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[2] S Kojima, et al. J Biol Chem. Midkine enhances fibrinolytic activity of bovine endothelial cells
Related Recombinant Proteins Products |
---|
Recombinant Human Serpin A12 (E.coli, N-GST)
Vaspin (Visceral Adipose-Specific SERPIN) is a newly described adipokine. Vaspin is also a unique insulin sensitizing adipocytokine in obesity. |
Recombinant Human B2M Protein (Mammalian, C-6His)
β-2-Microglobulin (B2M) is a secreted protein with 1 Ig-like C1-type (immunoglobulin-like) domain which belongs to the beta-2-microglobulin family. B2M may adopt the fibrillar configuration of amyloid in certain pathologic states. |
Recombinant Mouse E-Selectin/CD62E Protein (HEK293)
E-selectin, also known as endothelial leukocyte adhesion molecule-1 (ELAM-1) and CD62E, is an inducible adhesion molecule that is expressed on the surfaces of stimulated vascular endothelial cells and is sometimes involved in cancer cell metastasis. |
Recombinant Human DPP4/CD26 (Mammalian, C-6His)
DPP4/CD26 is a signal-anchor for type II membrane protein that belongs to the peptidase S9B family. DPP4/CD26 acts as a positive regulator of T-cell coactivation, by binding at least ADA, CAV1, IGF2R, and PTPRC. It’s binding to CAV1 and CARD11 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. |
Recombinant Human USP14 (E.coli, N-6His)
USP14 belongs to the ubiquitin-specific processing (USP) family which is a deubiquitinating enzyme (DUB) with His and Cys domains. USP14 acts also as a physiological inhibitor of endoplasmic reticulum-associated degradation (ERAD) under the non-stressed condition by inhibiting the degradation of unfolded endoplasmic reticulum proteins via interaction with ERN1. |
Products are for research use only. Not for human use. We do not sell to patients.
© Copyright 2010-2023 AbMole BioScience. All Rights Reserved.