Recombinant Mouse RANK (Receptor Activator of NF-kappa B) is produced by Mammalian expression system and the target gene encoding Val31-Ser214 is expressed with a 6His tag at the C-terminus. RANK can activate signaling pathways such as NF-κB, JNK, ERK, p38, and Akt/PKB, through TRAF protein phosphorylation after binding its ligand RANKL. RANK is ubiquitous highly expressed in trabecular bone, thymus, small intestine, lung, brain and kidney, but weakly expressed in spleen and bone marrow. RANK/TNFRSF11A signaling is largely considered to be growth promoting and apoptosis reducing such as the effects observed in osteoclasts. RANK/TNFRSF11A was also found to be involved in the regulation of interactions between T-cells and dendritic cells.
Accession: O35305
APMol Mass: 26-30 KDa, reducing conditions
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
| Solubility (25°C) | Dissolve the lyophilized protein in distilled water. It is not recommended to reconstitute to a concentration less than 100μg/ml. |
| Storage | Stored at ≤ -20°C, stable for one year after receipt |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[3] Jingxuan Yang, et al. Methods Mol Biol. Osteoclast Differentiation Assay
[4] Emilia Mia Sordillo, et al. Cancer. RANK-Fc: a therapeutic antagonist for RANK-L in myeloma
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