Species: Mouse
Expression system: HEK 293
Purity: SDS-PAGE, > 95%
Endotoxin: protein < 0.2 EU/μg was determined by gel coagulation
Apparent molecular weight: 22~ 27kda, sds-page under reduction conditions
Storage and stability: After receiving, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stable at 4°C for 1 week or -20°C for 3 months. For long-term preservation, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.
Biological activity: EC50 value of cardiac trophic factor-1 ≤1.25 ng/ mL and specific activity ≥0.8 × 106 units /mg were determined by dose-dependent proliferation of TF-1 cells. Ct-1 is associated with the pathophysiology of various heart diseases such as hypertension, myocardial infarction, valvular heart disease and congestive heart failure. This protein exerts its cellular effects by interacting with a glycoprotein 130 (GP130)/ leukemic suppressor receptor β (LIFR) heterodimer. Ct-1 activates cardiomyocyte phosphatidylinositol 3 kinase (PI-3 kinase) and enhances transcription factor NF-κB DNA-binding activity. Ct-1 is highly expressed in the heart, skeletal muscle, prostate and ovary, and low in lung, kidney, pancreas, thymus, testis and small intestine.
| Solubility (25°C) | It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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