Recombinant Human TGF-β1 (HEK293) regulates cell proliferation, growth, differentiation and cells movement. TGFβ is released from degranulating platelets and secreted by all of the major cell types participating in the repair process, including lymphocytes, macrophages, endothelial cells, smooth muscle cells, epithelial cells, and fibroblasts. Mammals express three isoforms of TGFβ designated TGFβI, TGFβ2, and TGFβ3; TGFβ1 is the most abundant isoform in all tissues, and in human platelets it is the only isoform of the peptide. TGFβ1 has immunomodulatory effects. TGFβ1 has profibrogenic effects.
The ED50 is ≤0.5 ng/mL as measured by HT-2 cells.
DNA sequence encoding human TGF-β1 (NP_000651.3) expressed with an Fc tag at the C-terminus.
Recombinant human TGF-β1 protein contains 374 amino acids with a predicted theoretical molecular weight of 42.2kD.
Endotoxin: <0.1EU/μg
Allergol Immunopathol (Madr). 2022 Mar 1;50(2):104-114.
6-Gingerol, a functional polyphenol of ginger, reduces pulmonary fibrosis by activating Sirtuin1
Recombinant Human TGF-β1 (HEK293, C-Fc) purchased from AbMole
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Source | Recombinant Human TGF-β1 (AbMole) |
| Method | SDS-PAGE analyses | |
| Cell Lines | ||
| Concentrations | ||
| Incubation Time | ||
| Results | The purity is > 97 % by SDS-PAGE analyses. |
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH2O or PBS up to 50 μg/ml. |
| Storage | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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