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Recombinant Human PD-1 (HEK293, His)

Cat. No. M21469
Recombinant Human PD-1 (HEK293, His) Structure
Synonym:

PDCD1、D279、SLEB2

Size Price Availability Quantity
100ug USD 750  USD750 In stock
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Quality Control
  • Current batch:
  • Purity >95%, Endotoxin < 0.1EU/μg
  • COA
  • MSDS
Biological Activity

Species: Human
Expression system: HEK293
Purity: > 95% by SDS-PAGE gel and high performance liquid chromatography
Endotoxin: < 0.1EU/μg
Molecular weight: Recombinant human PD-1 protein contains 148 amino acids and has a predicted theoretical molecular weight of 21.16kd
Storage and stability: Freeze-dried samples can be stored at 4℃ for 24 months, dissolved liquid can be stored at -20℃ for 6-12 months, and avoid repeated freeze-thawing



Biological activity: Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes. PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family. PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ. PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion. Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information
Molecular Weight
Formula
CAS Number
Purity >95%, Endotoxin < 0.1EU/μg
Solubility N/A
Storage at -20°C
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Keywords: Recombinant Human PD-1 (HEK293, His), PDCD1、D279、SLEB2 supplier, Cytokines, inhibitors

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