Recombinant Human Noggin Protein (HEK293) can bind to ligands of the transforming growth factor-β family and regulate its activity by inhibiting its pathway with signal receptors. Noggin was initially identified as a BMP-4 antagonist, and its effect is essential for the normal formation of the head and other back structures. Therefore, noggin has been shown to regulate the activities of other BMP-2, -7, -13 and -14. Transgenic mice overexpress noggin in mature osteoblasts, which is manifested by impaired differentiation of osteoblasts, decreased bone formation, and severe osteoporosis.
Determined by the ability of ATDC chondrocytes to inhibit the production of alkaline phosphatase by 5.0 ng/ml BMP-4, the expected ED50 of this effect is about 2.0-3.0 ng/ml.
The DNA sequence encoding human noggin (NP_005441.1) is expressed with a His tag at the C terminus.
Recombinant human noggin contains 211 amino acids and has a predicted theoretical molecular weight of 23.9 kd.
Endotoxin ≤0.01EU/ug
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH2O or PBS up to 100 μg/ml. |
| Storage | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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