Matrix metalloproteinase-8 (MMP-8) is a member of the peptidase M1A family. MMP-8 may affect the metastatic behavior of breast cancer cells through protection against lymph node metastasis, underlining the importance of anti-target identification in dгυg development.
MMP-8 plays essential roles in various physiological processes such as morphogenesis, differentiation, angiogenesis, and tissue remodeling, as well as pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases, and tumor invasion. MMP-8 participates in wound repair by contributing to the resolution of inflammation and open the possibility to develop new strategies for treating wound healing defects.
The recombinant human MMP8 consists of 458 amino acids after removal of the signal peptide. A DNA sequence encoding the human MMP8 (Met 1-Gly 467) was expressed with a polyhistidine tag at the C-terminus.
Measured by its ability to cleave the fluorogenic peptide substrate (Mca-PLGL-Dpa-AR-NH2), the specific activity is > 250 pmoles/min/μg.
Activation description: The proenzyme needs to be activated by APMA for an activated form.
Endotoxin < 1 EU/µg
Apparent Molecular Weight: 65-75 kDa, reducing conditions
Lyophilized from sterile 50mM Tris, 10mM CaCl2, 150mM NaCl, pH 7.5.
| Form | Lyophilized powder |
| Storage | Stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days and at -20°C for 3 months. |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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