Species: Human
Expression system: E. coli
Purity: > 95% by SDS-PAGE, > 95% HPLC analysis
Endotoxin: Protein content < 0.2 EU/μg determined by gel coagulation method
Apparent molecular weight: ~12.5 kDa, SDS-PAGE under reduction conditions
Storage and Stability: After receipt, the product remains stable at temperatures below -70°C for up to 6 months. After recombination, the product should be stabilized at 4°C for 1 week or at -20°C for 3 months. For long-term storage, carrier protein (e.g. 0.1% BSA) is recommended. Avoid repeated freeze-thaw cycles.
Bioactivity: Macrophage migration inhibitor (MIF) is a pleiotropic cytokine present in vivo as a homotrimer. MIF was originally identified as a T-cell-derived factor responsible for the inhibition of macrophage migration. In recent years, MIF has attracted much attention due to its possible role in angiogenesis and cancer development. MIF is overexpressed in a variety of cancers, including pancreatic, breast, colon, brain, prostate, skin, and lung cancers. The intratumoral expression of MIF is closely related to the expression of angiogenic growth factors, such as interleukin-8 (IL-8) and vascular endothelial growth factor (VEGF), which is closely related to the risk of recurrence after tumor resection.
| Solubility (25°C) | Reconstitute the lyophilized powder in ddH₂O up to 100 μg/ml. |
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[3] Qingqing Xiao, et al. Acta Pharm Sin B. Biological drug and drug delivery-mediated immunotherapy
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