Expression host: HEK293 cells
Molecular weight: Human CD19 Protein contains 271 amino acids and has a predicted theoretical molecular weight of 31.8 kD
Molecular aliases: CD19, B4, CVID3, MGC12802
Purity: ≥95% Was analyzed by SDS-PAGE gel and HIGH performance liquid chromatography
Endotoxin: < 0.1EU/μg
Storage and stability: Freeze-dried samples can be stored at 4℃ for 24 months, dissolved liquid can be stored at -20℃ for 6-12 months, and avoid repeated freeze-thaw
Bioactivity: The B lymphocyte antigen CD19, also known as Cluster of.erention 19(CD19), is a one-way type I membrane protein containing two ig-Likec2-type (.globulin-like) domains. CD19 was expressed in follicular dendritic cells and B cells. In fact, it is present on B cells, from early recognized B line cells during development to B cell mother cells, but lost to plasma cells at maturity. It primarily acts as a B cell co-receptor with CD21 and CD81. Upon activation, the cytoplasmic tail of CD19 is phosphorylated, leading to the binding of SrC-family kinases and recruitment of Pi-3 kinase. As with T cells, some surface molecules form antigenic receptors that form complexes on B lymphocytes. Most B-cell specific CD19 phosphoglycoproteins are one of these molecules, the others are CD21 and CD81. These surface immunoglobulin (sIg) -associated molecules promote signal transduction. On living B cells, anti-immunoglobulin antibodies mimic exogenous antigens to bind CD19 to and internalize sIg. The opposite process has not been confirmed, suggesting that the formation of this receptor complex is antigen-induced. This combination of molecules has been confirmed by chemical studies. Mutations in CD19 are associated with severe immunodeficiency syndrome, which is characterized by reduced antibody production. CD19 has been shown to interact with CD81, CD82, complement receptor 2, and VAV2.
| Storage |
Powder -20°C 3 years ; 4°C 2 years In solvent -80°C 6 months ; -20°C 1 month |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
[1] Ruihao Huang, et al. J Hematol Oncol. Recent advances in CAR-T cell engineering
[2] Carl H June, et al. N Engl J Med. Chimeric Antigen Receptor Therapy
[3] Michel Sadelain, et al. Nature. Therapeutic T cell engineering
[4] Kathrin Pieper, et al. J Allergy Clin Immunol. B-cell biology and development
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