Recombinant ASFV p72 complex is produced by Mammalian expression system and the target gene encoding Met1-Thr646&Met1-Leu530 is expressed with a 10His-3*Flag tag and a Flag-Strep tag at the N-terminus. Protein p72 is the major capsid protein of African swine fever virus (ASFV) and is an important target for test and vaccine development.
Accession: AXZ96139.1&AXZ96137.1
APMol Mass: 75-85&65-70kDa, reducing conditions
Supplied as a 0.2 μm filtered solution of 50mM HEPES，150mM NaCl，10% Glycerol，0.06% DDM，0.012% CHS，pH8.0.
| Storage | Stored at ≤ -20°C, stable for 12 months after receipt |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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