Puromycin dihydrochloride

Biological Activity

Puromycin dihydrochloride is a protein synthesis inhibitor. Puromycin 2HCl leads to the premature release of polypeptide chains as polypeptidyl purine derivatives. Analog of the 3' end of aminoacyl-tRNA. Puromycin dihydrochloride Inhibits translation in both in vitro and in vivo systems. Puromycin dihydrochloride inhibits the transport of proteins into the mitochondria in vitro. Puromycin inhibits protein import into mitochondria by interfering with an ATP-dependent step in the import process and that the ATP-dependent component in the reaction is located inside the inner mitochondrial membrane.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information

References

Puromycin inhibits protein import into mitochondria by interfering with an intramitochondrial ATP-dependent reaction.
Price J, et al. Biochim Biophys Acta. 1993 Jul 25;1150(1):89-97. PMID: 8334141.