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NBD-F is a fluorescent derivatization reagent for amino acid analysis.
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.
| Molecular Weight | 183.1 |
| Formula | C6H2FN3O3 |
| CAS Number | 29270-56-2 |
| Purity | >99% |
| Solubility | DMSO ≥ 60 mg/mL |
| Storage | 2-8°C, dry, protect from light, sealed |
Development of an LC-MS/MS method for the analysis of free sphingoid bases using 4-fluoro-7-nitrobenzofurazan (NBD-F).
Ishikawa T, et al. Lipids. 2014 Mar;49(3):295-304. PMID: 24310230.
Determination of amino acid neurotransmitters in rat hippocampi by HPLC-UV using NBD-F as a derivative.
Wu X, et al. Biomed Chromatogr. 2014 Apr;28(4):459-62. PMID: 24132719.
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