Nattokinase

Biological Activity

Nattokinase is an enzyme extracted and purified from a Japanese food called nattō. Nattokinase is a serine protease of the subtilisin family. It exhibits a strong fibrinolytic activity. A 2009 study showed that nattokinase may be effective in catabolism of toxic amyloid fibrils associated with Alzheimer's Disease, as well as the insulin fibrils associated with diabetes and the prion peptide fibrils associated with prion diseases.

Product Citations

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  Invest Ophthalmol Vis Sci. 2021 May 3;62(6):25.

  Nattokinase Attenuates Retinal Neovascularization Via Modulation of Nrf2/HO-1 and Glial Activation
  Nattokinase purchased from AbMole

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

Chemical Information

References

Production of nattokinase by batch and fed-batch culture of Bacillus subtilis.
Cho YH, et al. N Biotechnol. 2010 Sep 30;27(4):341-6. PMID: 20541632.