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Hydroxynaphthol Blue

Cat. No. M42854
Hydroxynaphthol Blue Structure
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Hydroxynaphthol Blue enables visual tube closure detection.

Chemical Information
Molecular Weight 620.47
Formula C20H11N2Na3O11S3
CAS Number 63451-35-4
Storage Powder          -20°C   3 years ;  4°C   2 years
In solvent       -80°C   6 months ;  -20°C   1 month
Conversion of different model animals based on BSA (PMID: 27057123)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.

References

[1] Xuliang Zhang et al. J Virol Methods. An optimized visual loop mediated isothermal amplification assay for efficient detection of minute virus of mice with hydroxynaphthol blue dye

[2] Qian Yang et al. Food Chem. Closed-tube saltatory rolling circle amplification with hydroxynaphthol blue for visual on-site detection of peanut as an allergenic food

[3] Shun Zhou et al. J Fish Dis. Rapid visual detection of Aeromonas salmonicida by loop-mediated isothermal amplification with hydroxynaphthol blue dye

[4] Naraporn Somboonna et al. Methods Mol Biol. Point-of-Care Chlamydia trachomatis Detection Using Loop-Mediated Isothermal Amplification and Hydroxynaphthol Blue

[5] I Choopara et al. Lett Appl Microbiol. Rapid and visual Chlamydia trachomatis detection using loop-mediated isothermal amplification and hydroxynaphthol blue

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