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H2DCFDA is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS) (Ex/Em=488/525 nm). H2DCFDA can be used for the Bacterial reactive oxygen species (ROS) production assay.
ROS Measurements
For the detection of intracellular ROS level ,ROS-sensitive probe H2DCFDA is used. Adherent cells (ESCs, difESCs, eMSCs, HeLa, U118) are incubated with 5 µM staining solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer. Lymphocytes, both control and PHA-activated, are resuspended in PBS, incubated with 5 µM of H2DCFDA in the dark for 30 min at 37°C, and immediately analyzed. Along with the H2DCFDA probe, if indicated, ROS-insensitive modification of the fluorescent dye DCFDA is used. The staining procedure is the same as for the H2DCFDA.
Mater Today Bio. 2025 Feb 03.
Nature-derived microneedles with metal-polyphenolic networks encapsulation for chronic soft tissue defects repair: Responding and remodeling the regenerative microenvironment
H2DCFDA purchased from AbMole
Science Advances. 2024 Aug 16;10(33):eado7249.
Magnetically attracting hydrogel reshapes iron metabolism for tissue repair
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J Colloid Interface Sci. 2024 Jun;663:810-824..
Amino acid-based metallo-supramolecular nanoassemblies capable of regulating cellular redox homeostasis for tumoricidal chemo-/photo-/catalytic combination therapy
H2DCFDA purchased from AbMole
GeroScience. 2024 Dec 27.
Paroxetine promotes longevity via ser-7-dop-4-IIS axis in Caenorhabditis elegans
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Int Immunopharmacol. 2024 Sep 12;142(Pt A):113041.
Salidroside alleviates ferroptosis in FAC-induced Age-related macular degeneration models by activating Nrf2/SLC7A11/GPX4 axis
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Foods. 2024 Dec 14.
Chemometric Classification and Bioactivity Correlation of Black Instant Coffee and Coffee Bean Extract by Chlorogenic Acid Profiling
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Plant Biotechnol J. 2023 May;21(5):943-960.
The C2H2-type zinc finger transcription factor OSIC1 positively regulates stomatal closure under osmotic stress in poplar
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Materials Today Bio. 2023 Jun 19.
Melanin inspired microcapsules delivering immune metabolites for hepatic fibrosis management
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J Agric Food Chem. 2023 Nov 22;71(46):18059-18073.
Transcriptome Analysis of GmPUB20A Overexpressing and RNA-Interferencing Transgenic Hairy Roots Reveals Underlying Negative Role in Soybean Resistance to Cyst Nematode
H2DCFDA purchased from AbMole
Int Immunopharmacol. 2023 Sep 21;124(Pt B):110963.
BNTA attenuates temporomandibular joint osteoarthritis progression by directly targeting ALDH3A1: An in vivo and in vitro study
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Burns Trauma. 2023 Oct 31;11:tkad030.
S100 calcium-binding protein A9 promotes skin regeneration through toll-like receptor 4 during tissue expansion
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Environ Toxicol. 2023 Jul 29.
Wogonin alleviates BaP-induced DNA damage and oxidative stress in human airway epithelial cells by dual inhibiting CYP1A1 activity and expression
H2DCFDA purchased from AbMole
Biochem Biophys Res Commun. 2023 Oct 5;682:163-173.
Experimental and theoretical studies on inhibition against tyrosinase activity and melanin biosynthesis by antioxidant ergothioneine
H2DCFDA purchased from AbMole
J Cosmet Dermatol. 2023 Jul 10.
Potent pigmentation inhibitory activity of incensole‐enriched frankincense volatile oil‐identification, efficacy and mechanism
H2DCFDA purchased from AbMole
Stem Cell Rev Rep. 2022 Aug;18(6):2088-2103.
Human Umbilical Cord-Derived Mesenchymal Stem Cells Ameliorate Skin Aging of Nude Mice Through Autophagy-Mediated Anti-Senescent Mechanism
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Parasit Vectors. 2022 Aug 1;15(1):274.
Neospora caninum infection induced mitochondrial dysfunction in caprine endometrial epithelial cells via downregulating SIRT1
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Comp Biochem Physiol C Toxicol Pharmacol. 2021 Apr;242:108938.
Clove essential oil confers antioxidant activity and lifespan extension in C. elegans via the DAF-16/FOXO transcription factor
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Ren Fail. 2021 Dec;43(1):900-910.
MMP-9-mediated regulation of hypoxia-reperfusion injury-related neutrophil inflammation in an in vitro proximal tubular cell model
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Mediators Inflamm. 2020 Jun 6;2020:4321912.
CD137 Signaling Promotes Endothelial Apoptosis by Inhibiting Nrf2 Pathway, and Upregulating NF- κ B Pathway
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Anat Rec (Hoboken). 2020 Jan 21.
Resibufogenin inhibited colorectal cancer cell growth and tumorigenesis through triggering ferroptosis and ROS production mediated by GPX4 inactivation
H2DCFDA purchased from AbMole
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Source | Comp Biochem Physiol C Toxicol Pharmacol (2021 Apr), Figure 3, H2DCFDA (Abmole Bioscience) |
| Method | measure the ROS levels | |
| Cell Lines | Each C. elegans strain | |
| Concentrations | 50 μM | |
| Incubation Time | 1 h | |
| Results | The results showed that CEO exposure made no significant difference to the GSH amount in each worm, however, the lipid peroxidation degree decreased due to the reduced ROS levels. |
| Molecular Weight | 487.29 |
| Formula | C24H16Cl2O7 |
| CAS Number | 4091-99-0 |
| Solubility (25°C) | DMSO: ≥ 90 mg/mL |
| Storage | -20°C, protect from light |
[1] Karyne Rangel, et al. Microorganisms. Detrimental Effect of Ozone on Pathogenic Bacteria
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