H2DCFDA is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS).
For the detection of intracellular ROS level ,ROS-sensitive probe H2DCFDA is used. Adherent cells (ESCs, difESCs, eMSCs, HeLa, U118) are incubated with 5 µM staining solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer. Lymphocytes, both control and PHA-activated, are resuspended in PBS, incubated with 5 µM of H2DCFDA in the dark for 30 min at 37°C, and immediately analyzed. Along with the H2DCFDA probe, if indicated, ROS-insensitive modification of the fluorescent dye DCFDA is used. The staining procedure is the same as for the H2DCFDA.
Ren Fail. 2021 Dec;43(1):900-910.
Mediators Inflamm. 2020 Jun 6;2020:4321912.
CD137 Signaling Promotes Endothelial Apoptosis by Inhibiting Nrf2 Pathway, and Upregulating NF- κ B Pathway
H2DCFDA purchased from AbMole
Comp Biochem Physiol C Toxicol Pharmacol. 2021 Apr;242:108938.
Anat Rec (Hoboken). 2020 Jan 21.
|Source||Comp Biochem Physiol C Toxicol Pharmacol (2021 Apr), Figure 3, H2DCFDA (Abmole Bioscience)|
|Method||measure the ROS levels|
|Cell Lines||Each C. elegans strain|
|Incubation Time||1 h|
|Results||The results showed that CEO exposure made no significant difference to the GSH amount in each worm, however, the lipid peroxidation degree decreased due to the reduced ROS levels.|
|Body Surface Area (m2)||0.007||0.025||0.15||0.05||0.02||0.5|
|Animal A (mg/kg) = Animal B (mg/kg) multiplied by||Animal B Km|
|Animal A Km|
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
|Solubility||DMSO: ≥ 90 mg/mL|
|Storage||-20°C, protect from light|
Redox environment in stem and differentiated cells: A quantitative approach.
Lyublinskaya OG, et al. Redox Biol. 2017 Aug;12:758-769. PMID: 28426982.
Reactive Oxygen Species in Planarian Regeneration: An Upstream Necessity for Correct Patterning and Brain Formation
Nicky Pirotte, et al. Oxid Med Cell Longev. 2015;2015:392476. PMID: 26180588.
Sustained production of ROS triggers compensatory proliferation and is required for regeneration to proceed
Carole Gauron, et al. Sci Rep. 2013;3:2084. PMID: 23803955.
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