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Forchlorfenuron

Cat. No. M8890
Forchlorfenuron Structure
Synonym:

FCF, KT-30, CPPU

Size Price Availability Quantity
500mg USD 150 In stock
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Quality Control
Biological Activity

Forchlorfenuron (FCF) is a synthetic cytokinin that inhibits septins and exhibits anti-parasitic, anticancer, and anti-angiogenic activities. Forchlorfenuron is also a plant growth regulator. Cytotoxicity of forchlorfenuron and its metabolites are tested through Sulforhodamine B assays against CHO cells. Forchlorfenuron exhibits significant cytotoxicity against CHO cells with an IC50 of 12.12±2.14 μM.

In live mammalian cells, FCF dampens septin dynamics and induces the assembly of abnormally large septin structures. FCF has a low level of cytotoxicity, and these effects are reversed upon FCF washout. Significantly, FCF treatment induces mitotic and cell migration defects that phenocopy the effects of septin depletion by small interfering RNA. FCF targets the filamentous assembly of divergent septin isoforms (e.g. Cdc3/10/11/12 (42), SEPT2, SEPT7) reversibly and across species.

Forchlorfenuron (FCF) has low oral toxicity in rats with an LD50 of 4940 mg/kg bw in male rats and 4899 mg/kg bw in females.

Protocol
Cell Experiment
Cell lines HeLa or MDCK cells
Preparation method HeLa or MDCK cells are plated overnight on collagen-coated 35-mm tissue culture dishes and then treated with DMSO or FCF for 4 h. Cells are trypsinized, resuspended in DMEM supplemented with 10% FBS, and mixed in a 1:1 ratio with trypan blue at room temperature for 1 min. Blue and non-blue cells are counted using a hemocytometer.
Concentrations 6.25-500 μM
Incubation time 4 h
Animal Experiment
Animal models toxicity test of forchlorfenuron in rats
Formulation -
Dosages 4940 mg/kg bw in male rats and 4899 mg/kg bw in females
Administration oral
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 247.68
Formula C12H10ClN3O
CAS Number 68157-60-8
Purity 99.64%
Solubility DMSO: 30 mg/mL
Storage at -20°C
References

Transformation products elucidation of forchlorfenuron in postharvest kiwifruit by time-of-flight mass spectrometry.
Zhang Z, et al. PLoS One. 2017 Sep 6;12(9):e0184021. PMID: 28877224.

Forchlorfenuron alters mammalian septin assembly, organization, and dynamics.
Hu Q, et al. J Biol Chem. 2008 Oct 24;283(43):29563-71. PMID: 18713753.

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Keywords: Forchlorfenuron, FCF, KT-30, CPPU supplier, inhibitors

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