Evans Blue

Biological Activity

Evans blue is a biological dye, for detection of cell survival. Evans Blue can not penetrate healthy cell membrane, but can penetrate into death cell. Evans Blue is also a potent inhibitor of L-glutamate uptake via the membrane bound excitatory amino acid transporter (EAAT). It is able to antagonize AMPA and kainate receptor mediated currents with IC50 values of 220 and 150 nM, respectively.

Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)

Species	Mouse	Rat	Rabbit	Guinea pig	Hamster	Dog
Weight (kg)	0.02	0.15	1.8	0.4	0.08	10
Body Surface Area (m2)	0.007	0.025	0.15	0.05	0.02	0.5
Km factor	3	6	12	8	5	20

Animal A (mg/kg) = Animal B (mg/kg) multiplied by	Animal B Km
	Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the K_m factor for a mouse and then divide by the K_m factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information

References

Neuroprotective Effects of Metformin on Traumatic Brain Injury in Rats Is Associated With the AMP-activated Protein Kinase Signaling Pathway
Siavash Rahimi, et al. Metab Brain Dis. 2020 Jul 3. PMID: 32621159.

The Effect of O-1602, a GPR55 Agonist, on the Cyclophosphamide-Induced Rat Hemorrhagic Cystitis
Andrzej Wrobel, et al. Eur J Pharmacol. 2020 Jun 29;173321. PMID: 32615180.

Haem Oxygenase Protects Against Thrombocytopaenia and Malaria-Associated Lung Injury
Isaclaudia G de Azevedo-Quintanilha, et al. Malar J. 2020 Jul 1;19(1):234. PMID: 32611348.