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Doxorubicin HCL

Cat. No. M1969
Doxorubicin HCL Structure
Synonym:

Adriamycin, Rubex

Size Price Availability Quantity
50mg USD 120 In stock
100mg USD 200 In stock
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Quality Control
Biological Activity

Doxorubicin (Adriamycin) is an antineoplastic agent used in the treatment of a wide range of cancers, including hematological malignancies, many types of carcinoma, and soft tissue sarcomas. Doxorubicin interacts with DNA by intercalation and inhibition of macromolecular biosynthesis. Doxorubicin stabilizes the topoisomerase II complex after it has broken the DNA chain for replication, preventing the DNA double helix from being resealed and thereby stopping the process of replication. In studies with isolated nuclei, adriamycin was also a more potent inhibitor of DNA synthesis than RNA synthesis. However, with intact cells, adriamycin inhibited both DNA and RNA synthesis to about the same extent. The inhibition produced by adriamycin on RNA synthesis in intact cells was greater than that observed in the cell-free systems. Adriamycin inhibited protein synthesis in a cell-free system consisting of polyribosomes, transfer RNA, and enzymes but did not inhibit protein synthesis in intact cells. Adriamycin-induced congestive heart failure (CHF) can best be prevented by limiting the total dose as follows: 400 to 450 mg per sq meter following mediastinal radiation and 500 to 550 mg per sq meter for patients without other significant risk factors. Combination therapy experiments with sirolimus (rapamycin) and doxorubicin have shown promise in treating Akt-positive lymphomas in mice.

Protocol
Cell Experiment
Cell lines Human choriocarcinoma cell line
Preparation method Briefly, cells were plated on 96-well plates (10,000 cells/well) and a day after (cell culture 80% confluent) were exposed to doxorubicin (0–8 mM), L-DOX (0–8 mM) or PL-DOX (0–200 mM) in non-supplemented serum-free growth medium for 4 h. After exposure, the cells were washed and further incubated for 20 h.
Concentrations 0–8 µM
Incubation time 24 h
Animal Experiment
Animal models Male Sprague-Dawley rats
Formulation
Dosages 4 mg/kg
Administration i.v.
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 579.98
Formula C27H29NO11.HCl
CAS Number 25316-40-9
Purity >99%
Solubility DMSO 100 mg/mL
Storage at -20°C
Product Citations
References

Cellular pharmacokinetic mechanisms of adriamycin resistance and its modulation by 20(S)-ginsenoside Rh2 in MCF-7/Adr cells.
Zhang J, et al. Br J Pharmacol. 2012 Jan;165(1):120-34. PMID: 21615726.

Effect of adriamycin on DNA, RNA, and protein synthesis in cell-free systems and intact cells.
Momparler RL, et al. Cancer Res. 1976 Aug;36(8):2891-5. PMID: 1277199.

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  Catalog
Abmole Inhibitor Catalog 2017




Keywords: Doxorubicin HCL, Adriamycin, Rubex supplier, Topoisomerase, inhibitors

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