Digoxin is a potent inhibitor of Na+/K+-ATPase, has the potential for arrhythmia and heart failure.
Cell Experiment | |
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Cell lines | A549 cells |
Preparation method | Cells were treated with various concentrations of digoxin for 24 h and seeded in transwell chambers (8-μm pore size, 6.5-mm diameter), which were or were not coated with Matrigel. The upper wells were filled with serum-free media and A549 cells (2×104 or 1×104 cells per well). The lower wells of the transwells contained the same media with 10% FBS and digoxin at various concentrations. |
Concentrations | 250 nM |
Incubation time | 24 h |
Animal Experiment | |
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Animal models | Sprague Dawley Rats |
Formulation | 0.5% sodium carboxymethylcellulose |
Dosages | 25.6 μmol/kg |
Administration | oral gavage |
Molecular Weight | 780.94 |
Formula | C41H64O14 |
CAS Number | 20830-75-5 |
Solubility (25°C) | DMSO 40 mg/mL |
Storage | -20°C, protect from light, sealed |
Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
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