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Digoxin

Cat. No. M3935
Digoxin Structure
Size Price Availability Quantity
200mg USD 100 In stock
500mg USD 150 In stock
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Quality Control
Biological Activity

Digoxin is a widely used compound for ventricular rate control in patients with atrial fibrillation (AF), despite a scarcity of randomised trial data.Digoxin treatment was associated with a significant increase in all-cause mortality, vascular death, and sudden death in patients with AF. This association was independent of other measured prognostic factors, and although residual confounding could account for these results, these data show the possibility of digoxin having these effects. A randomised trial of digoxin in treatment of AF patients with and without heart failure is needed.

Protocol
Cell Experiment
Cell lines A549 cells
Preparation method Cells were treated with various concentrations of digoxin for 24 h and seeded in transwell chambers (8-μm pore size, 6.5-mm diameter), which were or were not coated with Matrigel. The upper wells were filled with serum-free media and A549 cells (2×104 or 1×104 cells per well). The lower wells of the transwells contained the same media with 10% FBS and digoxin at various concentrations.
Concentrations 250 nM
Incubation time 24 h
Animal Experiment
Animal models Sprague Dawley Rats
Formulation 0.5% sodium carboxymethylcellulose
Dosages 25.6 μmol/kg
Administration oral gavage
Conversion of different model animals based on BSA (Value based on data from FDA Draft Guidelines)
Species Mouse Rat Rabbit Guinea pig Hamster Dog
Weight (kg) 0.02 0.15 1.8 0.4 0.08 10
Body Surface Area (m2) 0.007 0.025 0.15 0.05 0.02 0.5
Km factor 3 6 12 8 5 20
Animal A (mg/kg) = Animal B (mg/kg) multiplied by  Animal B Km
Animal A Km

For example, to modify the dose of resveratrol used for a mouse (22.4 mg/kg) to a dose based on the BSA for a rat, multiply 22.4 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for resveratrol of 11.2 mg/kg.

Chemical Information
Molecular Weight 780.94
Formula C41H64O14
CAS Number 20830-75-5
Purity >99%
Solubility
Storage at -20°C
Customer Product Validations & Biological Datas
Source Nature (2011). Figure 4. Digoxin
Method Flow cytometry
Cell Lines Th17 cells
Concentrations 10 μM
Incubation Time 6 d
Results In vitro digoxin treatment of expanded mouse Th17 cells derived from immunized mice inhibited both IL-23R (Fig. 4a) and IL-17a expression without affecting IFN-γ expression (Fig. 4b).
Rating
Source Nature (2011). Figure 1. Digoxin
Method luciferase reporter
Cell Lines Drosophila S2 cell
Concentrations 10 μM
Incubation Time
Results Inhibition of RORγ activity by digoxin was specific, as there was no effect on the transcriptional activity of VP16 or of the related nuclear hormone receptors RORα and DHR3
Rating
References

Digoxin use and risk of mortality in hypertensive patients with atrial fibrillation.
Okin PM, et al. J Hypertens. 2015 Mar 19. PMID: 25799208.

Digoxin use in patients with atrial fibrillation and adverse cardiovascular outcomes: a retrospective analysis of the Rivaroxaban Once Daily Oral Direct Factor Xa Inhibition Compared with Vitamin K Antagonism for Prevention of Stroke and Embolism Trial in Atrial Fibrillation (ROCKET AF).
Washam JB, et al. Lancet. 2015 Mar 5. PMID: 25749644.

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