DFHBI 1T is a mimic of green fluorescent protein (GFP) fluorophore for imaging RNA in living cells. Fluorescence activated by binding to Spinach2 or Broccoli aptamers. Exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. Exhibits higher specific fluorescence and lower background fluorescence when bound to Spinach2 compared with Spinach2-DFHBI.
| Molecular Weight | 320.21 |
| Formula | C13H9F5N2O2 |
| CAS Number | 1539318-36-9 |
| Form | Solid |
| Solubility (25°C) | DMSO 40 mg/mL |
| Storage | -20°C, protect from light |
| Species | Mouse | Rat | Rabbit | Guinea pig | Hamster | Dog |
| Weight (kg) | 0.02 | 0.15 | 1.8 | 0.4 | 0.08 | 10 |
| Body Surface Area (m2) | 0.007 | 0.025 | 0.15 | 0.05 | 0.02 | 0.5 |
| Km factor | 3 | 6 | 12 | 8 | 5 | 20 |
| Animal A (mg/kg) = Animal B (mg/kg) multiplied by | Animal B Km |
| Animal A Km |
For example, to modify the dose of Compound A used for a mouse (20 mg/kg) to a dose based on the BSA for a rat, multiply 20 mg/kg by the Km factor for a mouse and then divide by the Km factor for a rat. This calculation results in a rat equivalent dose for Compound A of 10 mg/kg.
| Related Nucleic Acid Staining Products |
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| Cyanine 5 Tyramide methyl indole
Cyanine 5 Tyramide (Tyramide-Cy5) methyl indole is a red fluorescent dye. |
| 2-Aminopurine
2-Aminopurine has been used to inhibit eukaryotic initiation factor-2α (eIF2α)-phosphorylation of osteoarthritis (OA) chondrocytes. 2-Aminopurine, a fluorescent analog of guanosine and adenosine, is a widely used fluorescence-decay-based probe of DNA structure. 2-Aminopurine has been used to probe nucleic acid structure and dynamics. |
| 7-Aminoactinomycin D
7-aminoactinomycin D (7-aad) can bind to DNA, and the maximum excitation/emission wavelength is 546nm/647 nm. The best excitation laser is 561nm or 488nm. It gives red fluorescence and is often used to observe the nucleus and nucleic acid. Store in freezer (-5 to -30°C) away from light. The solvent was DMSO. *The compound is unstable in solutions, freshly prepared is recommended |
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